behavior/neurological
• abnormal hind-limb clasping reflex at 3 months of age
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• mice show reduced latency to fall on the rotarod at 3 months of age
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• during locomotion, most mice show very low position of the body and dragging tail
• mice show more narrow gait angles and shorter stride length at 5 and 12 months of age
• 12 month old mice show a decrease of toe spreading and an increase in extension of plantar length during locomotion
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• shorter stride length in 5 and 12 months old mice
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nervous system
• embryonic motor neuron cultures show reduced axon length growth at 48 hours
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• embryonic motor neuron cultures show reduced axon length growth at 48 hours
• cultured motor neurons show abnormal organelles structures including larger mitochondria and increased numbers of various sized vacuoles
• mitochondrial interconnectivity is reduced in the proximal segment of motor neuron axons after 24 and 48 hours in culture
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• motor neurons in the anterior horn show cell lesions such as vacuoles and chromatolysis at 5 and 12 months of age
• motor neuron degeneration in the anterior horn occurs in the first months of life
• however, mice do not show a reduction in axonal number or myelin thickness and distribution of axon sizes in both proximal and distal sciatic nerves is normal
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• dorsal root ganglia sensory neurons from 5 month old mice generate neurites with reduced average length when grown in culture
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• occupancy of neuromuscular junctions (percentage of postsynaptic buttons that are completely filled by the nerve terminal) is reduced in 12 month old, but not 5 month old, mice
• muscles show abnormal tangle-like structures at terminal axons close to the neuromuscular junction
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• motor neurons in the anterior horn show cell lesions such as vacuoles and chromatolysis at 5 and 12 months of age
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• dorsal root ganglia sensory neurons from 5 month old mice generate neurites with reduced average length when grown in culture
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• 5 month old, but not 2 month old, mice show a reduction of compound muscle action potential amplitude for distal (at the ankle) and proximal (at the hip) stimulation, indicating axonal neuropathy
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• 5 month old mice show a slight but significant reduction in motor nerve conduction velocity
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• the number of mitochondria is increased at 24 hours in culture in the proximal segment of motor neuron axons, indicating a delay of mitochondrial distribution along the axon which normalized by 48 hours in culture
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homeostasis/metabolism
• cultured motor neurons show reduced cytosolic calcium and reduction in calcium inflow through store-operated calcium entry upon mobilization of endoplasmic reticulum (ER) calcium by thapsigargin
• motor neurons also show slow entrance velocity of calcium into the neuron and slow entry of calcium after store calcium depletion
• neurons show a lower increase of cytoplasmic calcium when treated with ionomycin to release calcium from cellular stores
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limbs/digits/tail
• some mice show everted paws
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cellular
• cultured embryonic motor neurons show changes in ER cristernae
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• cultured motor neurons show larger and abnormal mitochondria
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• embryonic motor neuron cultures show reduced axon length growth at 48 hours
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
Charcot-Marie-Tooth disease axonal type 2K | DOID:0110167 |
OMIM:607831 |
J:224701 |