growth/size/body
• although normal up to 8 months of age, body weight is reduced by 17% at 16 months of age
|
• enlarged bladder at post mortem after 16 months of age
|
behavior/neurological
N |
• no obvious learning and memory deficits noted in the Morris water maze at 5 months of age
|
• progressive decline in beam walking performance, starting at 8 months of age
• significantly impaired motor coordination at 16 months, when homozygotes fall off the beam in about 1 of 3 trials
|
• at 16 months of age
|
• progressive gait disorder and motor deficits starting at 12 months of age, as quantified by the foot-base-angle at toe-off positions of the hindpaws
• at 16 months of age, the foot-base-angle is reduced to ~50 degrees versus ~75 degrees in wild-type mice
|
nervous system
• after 4 days in culture, the length of outgrowing axons as well as the bidirectional axonal transport rate of mitochondria are significantly decreased in mutant embryonic motoneurons
• however, axonal branching is not altered in cultured motoneurons
|
• significantly reduced brain weight at 16 months of age
|
• Purkinje cells severely reduced to 26% of wild-type controls at 16 months of age, consistent with the ataxic gait disorder
|
• significantly reduced brain size at 16 months of age
• however, normal brain structures and weight at 2 months of age
• no thinning of the corpus callosum at 16 months of age
|
astrocytosis
(
J:223127
)
• marked astrogliosis in the deep layers V and VI of the motor cortex and in the cerebellum at 16 months, but not at 2 months of age
• clear activation of astrocytes in the spinal cord (mainly in the white matter) at 16 months, but not at 2 months of age
• however, no astrogliosis observed in the hippocampus or the olfactory bulb
|
• significantly reduced number of neurons in deep layers V and VI of the motor cortex at 16 months of age
• however, normal number of neurons in the motor cortex at 2 and 8 months of age
• no neurodegeneration observed in the hippocampus or the olfactory bulb
|
• axonal degeneration in the corticospinal tract at 8 months, but not at 2 months of age
• large diameter axons are almost absent from horizontal lumbar spinal cord sections at 16 months of age
|
renal/urinary system
• enlarged bladder at post mortem after 16 months of age
|
• dysfunction of the bladder at 16 months of age
|
cellular
• pathological accumulations of autofluorescent, electron-dense material in lysosomal structures of mutant neurons, preceding degeneration of cortical
motoneurons and Purkinje cells
|
• after 4 days in culture, the length of outgrowing axons as well as the bidirectional axonal transport rate of mitochondria are significantly decreased in mutant embryonic motoneurons
• however, axonal branching is not altered in cultured motoneurons
|
• altered composition/function of lysosomes
• increased enzymatic activities of lysosomal enzymes beta-hexosaminidase and beta-galactosidase in brain at 16 months, but not at 2 months of age
|
skeleton
Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
hereditary spastic paraplegia 15 | DOID:0110768 |
OMIM:270700 |
J:223127 |