Analysis Tools
pigmentation
 |
• at P5.5, mice show significantly fewer pigmented or LacZ+ hair follicles than control mice; also, completely undifferentiated hair follicles that are neither pigmented nor LacZ+ are observed, unlike in control mice
• at P5.5, the total number of melanocytes (S100b+) per hair follicle is reduced by 40% relative to that in control mice
• at P5.5, mice exhibit formation of undifferentiated melanocytes in the bulge area of hair follicles, along with loss of MITF (the master regulator of melanocyte development) and upregulation of ZEB1 expression
• the number of MITF+ melanocytes is strongly reduced relative to the total number of melanocytes per hair follicle (S100b+ melanocytes), whereas the number of PAX3+ melanocytes is normal; also, MITF protein levels are reduced in MITF+ cells
• most hair follicles are negative for terminal melanocyte differentiation markers, such as TYRP1 and tyrosinase enzymatic activity
• mRNA expression of several melanocyte differentiation markers (Tyrp1,Tyr, Dct, Pmel, Mc1R and Mitf) is significantly down-regulated in skin at E15.5
|
|
|
• at P5.5, a few remaining melanosomes are visible in some hair follicles; however, these melanosomes are spherical with irregular borders, unlike the rod-shaped melanosomes of control hair follicles
|
|
|
• at P5.5, ZEB2-stained sections revealed absence of melanin in most melanocytes of the bulb area
|
|
|
• at P5.5, ZEB2-stained sections revealed absence of melanin in the hair shafts
|
integument
|
|
• at P5.5, mice show significantly fewer pigmented or LacZ+ hair follicles than control mice; also, completely undifferentiated hair follicles that are neither pigmented nor LacZ+ are observed, unlike in control mice
• at P5.5, the total number of melanocytes (S100b+) per hair follicle is reduced by 40% relative to that in control mice
• at P5.5, mice exhibit formation of undifferentiated melanocytes in the bulge area of hair follicles, along with loss of MITF (the master regulator of melanocyte development) and upregulation of ZEB1 expression
• the number of MITF+ melanocytes is strongly reduced relative to the total number of melanocytes per hair follicle (S100b+ melanocytes), whereas the number of PAX3+ melanocytes is normal; also, MITF protein levels are reduced in MITF+ cells
• most hair follicles are negative for terminal melanocyte differentiation markers, such as TYRP1 and tyrosinase enzymatic activity
• mRNA expression of several melanocyte differentiation markers (Tyrp1,Tyr, Dct, Pmel, Mc1R and Mitf) is significantly down-regulated in skin at E15.5
|
|
|
• at P5.5, ZEB2-stained sections revealed absence of melanin in most melanocytes of the bulb area
|
|
|
• at P5.5, ZEB2-stained sections revealed absence of melanin in the hair shafts
|
|
|
• mice show a reduction in LacZ+ melanocyte stem cells in the bulge area relative to control mice, consistent with fewer melanoblasts found in the epidermis
|
embryo
|
|
• at E15.5, the number of LacZ+ melanoblasts in the epidermis of both the belly and the back is significantly lower than that in control embryos; however, 30% of them reach the dorsal area (back) at E15.5
• at P5.5, some hair follicles are still LacZ+ and/or (hypo-)pigmented, indicating that melanoblasts can migrate and populate the bulb area of the hair follicles
• the number of melanoblasts present in the dermis at E15.5 is normal
|
|
|
• at E15.5, the number of LacZ+ melanoblasts is significantly reduced in both dorsal and ventral areas relative to control embryos; melanoblast numbers are reduced more on the belly (95%) than on the back (70%), consistent with a migration defect
• at E15.5, melanoblasts exhibit less cell protrusions than control cells
|
nervous system
|
|
• at E15.5, the number of LacZ+ melanoblasts is significantly reduced in both dorsal and ventral areas relative to control embryos; melanoblast numbers are reduced more on the belly (95%) than on the back (70%), consistent with a migration defect
• at E15.5, melanoblasts exhibit less cell protrusions than control cells
|
cellular
|
|
• at E15.5, the number of LacZ+ melanoblasts in the epidermis of both the belly and the back is significantly lower than that in control embryos; however, 30% of them reach the dorsal area (back) at E15.5
• at P5.5, some hair follicles are still LacZ+ and/or (hypo-)pigmented, indicating that melanoblasts can migrate and populate the bulb area of the hair follicles
• the number of melanoblasts present in the dermis at E15.5 is normal
|
