Phenotypes Associated with This Genotype

Genotype
MGI:6294898

• Allelic Composition: Nlgn2^tm1.1Gchn/Nlgn2^tm1.1Gchn
• Genetic Background: involves: 129S/SvEv * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:272181 )

• in the contextual fear conditioning test, mice are capable of associating the conditioning chamber with foot-shock in the initial training but they fail to retain the fear context memory in the following days of testing, indicating impaired hippocampal dependent cognitive function

abnormal spatial working memory ( J:272181 )

• mice show reduced spontaneous alternation in the Y maze, indicating a working memory dysfunction

abnormal emotion/affect behavior ( J:272181 )

• in an acute stress test in which mice are placed into restraining tubes for 1 hour, mutants struggle more intensively for a long time and excrete more than wild-type mice

increased thigmotaxis ( J:272181 )

• mice spend less time in the center region during an open field test, although the total distance traveled is similar to wild-type mice

• in the elevated plus maze test, mice spend less time in the open arm than wild-type mice, while the total travel distance is similar to wild-type mice

decreased freezing behavior ( J:272181 )

• mice show a reduction of freezing time in the forced swim test indicating hyperactivity induced by acute stress

decreased startle reflex ( J:272181 )

• mice show a reduction in the startle response when stimulated at 100-120 dB

induced hyperactivity ( J:272181 )

• mice show a reduction of freezing time in the forced swim test indicating hyperactivity induced by acute stress

homeostasis/metabolism

increased circulating corticosterone level ( J:272181 )

• mice show a much higher level of corticosterone after restraining in the acute stress test, indicating a hyperactive hypothalamic-pituitary-adrenal axis response towards stress

nervous system

abnormal postsynaptic density morphology ( J:272181 )

• mice show a reduction in GABAergic synapse density, with a decrease of postsynaptic GABA(A) receptor gamma2 subunit and the scaffold protein gephyrin around cell soma in hippocampal regions and reduced parvalbumin+ and vesicular GABA transporter (vGAT)+ puncta number and size in the dentate granule cells and CA1/CA3 pyramidal cells

abnormal hippocampus physiology ( J:272181 )

• mice show a decrease of cFos-positive cells in the dentate gyrus and CA2/3 regions of the hippocampus after stress indicating impaired hippocampal activation toward acute stress

abnormal miniature inhibitory postsynaptic currents ( J:272181 )

• both the frequency and amplitude of miniature inhibitory postsynaptic currents (mIPSCs) are decreased in dentate granule cells indicating impaired GABAergic neurotransmission

• however, no change is seen in miniature excitatory postsynaptic currents (mEPSCs) in dentate granule cells

decreased miniature inhibitory postsynaptic current amplitude ( J:272181 )

• in dentate granule cells

decreased miniature inhibitory postsynaptic current frequency ( J:272181 )

• in dentate granule cells

decreased prepulse inhibition ( J:272181 )

• pre-pulse inhibition is impaired

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
schizophrenia		DOID:5419	OMIM:181500	J:272181