Phenotypes Associated with This Genotype

Genotype
MGI:6358417

• Allelic Composition: Nsmf^tm1.2Mrkr/Nsmf^tm1.2Mrkr
• Genetic Background: involves: C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:232404 )

• mice are impaired in contextual fear conditioning

• however, cued fear conditioning is not affected and mice exhibit normal auditory discrimination learning

abnormal object recognition memory ( J:232404 )

• mice are impaired in object recognition memory

increased locomotor activity ( J:232404 )

• mice are hyperactive in the open field, with mice spending more time with locomotor activity during the test period

digestive/alimentary system

increased defecation amount ( J:232404 )

• mice produce a higher number of fecal boli during odor exposition

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:232404 )

♂N • males do not exhibit gonadal defects; testes are unaltered and no differences in various stages of differentiation from spermatogonia to sperm cells is seen

decreased primary ovarian follicle number ( J:232404 )

♀

decreased secondary ovarian follicle number ( J:232404 )

♀ • number of secondary follicles, but not corpora lutea, is slightly reduced

decreased tertiary ovarian follicle number ( J:232404 )

♀ • lower numbers of tertiary follicles

homeostasis/metabolism

decreased circulating estradiol level ( J:232404 )

♀ • estradiol levels are reduced in the estrus and metestrus phase

♀ • however, plasma levels of luteinizing hormone and progesterone are normal throughout the estrous cycle

nervous system

abnormal striatum morphology ( J:232404 )

• small, but significant, increase in the volume of the striatum

abnormal hippocampus morphology ( J:232404 )

• hippocampal dysplasia with a reduced number of synapses

• however, the number of GnRH+ neurons and fibers are normal in the brain, and general brain morphology is not altered

abnormal innervation ( J:232404 )

• altered catecholaminergic innervation of the hippocampus with an increase in the number of tyrosine-hydroxylase positive fivers in the CA3 but not CA1 region or dentate gyrus

• however, catecholaminergic innervation in the striatum and ventral tegmental area is normal

abnormal dendrite morphology ( J:232404 )

• reduction of dendritic complexity in CA1 neurons, with apical and more prominently basal dendrites are affected

• mice exhibit shorter dendrites and much lower number of branches for a given dendritic segment

• hippocampal primary neuron cultures show a simplified dendritic tree, with shorter neurite length and less branching of MAP2+ dendrites and reduced number of synaptic contacts at day in vitro 15

decreased dendritic spine density ( J:232404 )

• spine density is reduced in basal and apical dendrites in secondary branches of CA1 neurons

• hippocampal primary neuron cultures show a reduced number of spines during neuronal development

abnormal synapse morphology ( J:232404 )

• reduction in number of synapses in the hippocampus

impaired synaptic plasticity ( J:232404 )

• mice show reduced synaptic plasticity of hippocampal CA1 synapses in adulthood

abnormal excitatory postsynaptic potential ( J:232404 )

• mice exhibit a decay of the field excitatory postsynaptic potential (fEPSP) slope already 30-40 minutes after the induction of long term potentiation (LTP)

• however, basal synaptic transmission, paired-pulse facilitation and the input-output curve are not changed

reduced long-term potentiation ( J:232404 )

• mice show reduced hippocampal long-term potentiation at CA1 synapses, with late phase LTP almost absent despite a similar induction of early LTP as in wild-type mice

reproductive system

reproductive system phenotype ( J:232404 )

N • hypogonadotropic hypogonadism or subfertility are not seen

decreased primary ovarian follicle number ( J:232404 )

♀

decreased secondary ovarian follicle number ( J:232404 )

♀ • number of secondary follicles, but not corpora lutea, is slightly reduced

decreased tertiary ovarian follicle number ( J:232404 )

♀ • lower numbers of tertiary follicles

short diestrus ( J:232404 )

♀ • females exhibit shortening of the diestrus phase

♀ • however, duration of the proestrus and estrus phase are not different

prolonged metestrus ( J:232404 )

♀ • females exhibit prolonged duration of the metestrus phase

taste/olfaction

taste/olfaction phenotype ( J:232404 )

N • mice do not show signs of anosmia or hyposmia and have a normal organization of the bulbus olfactorius

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	Kallmann syndrome	DOID:3614		J:232404