Phenotypes Associated with This Genotype

Genotype
MGI:6403118

• Allelic Composition: Tg(DPP4)2Nnag/0
• Genetic Background: B6.Cg-Tg(DPP4)2Nnag

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal cytokine level ( J:286321 )

• MERS-CoV-infected mice (both young and adults) show increased expression of proinflammatory cytokines and chemokines in the lungs, including IP-10, IL-6, IL-13, MCP-1, MIP-1alpha, MIG, IL-12, and IFN-gamma, with higher levels of IP-10, IL-12, and IL-1beta in adult mice and IL-1alpha and IL-17 detected only in the adult mice, and a transient increase in IFN-alpha4 and IFN-beta in adults, indicating a more severe reaction in aged mice

lung inflammation ( J:286321 )

• 10 week old mice infected with MERS-CoV show progressive pulmonary inflammation and develop acute pneumonia from which they recover; inflammatory reactions include partial and/or mild perivascular and peribronchiolar infiltration by mononuclear cells and eosinophils in alveolar areas on 3 and 5 dpi, and severe lung inflammation, including perivascular and alveolar septal thickening causing by infiltrating mononuclear cells on 7 dpi, clearing of inflammatory responses is seen by 35 dpi although focal cellular infiltration is still seen in the peribronchioles and alveolar septa and no inflammation is seen in the brain through 35 dpi

• 25 week old mice infected with MERS-CoV show delayed and prolonged inflammatory responses in the lung compared to 10 week old mice

increased susceptibility to Coronaviridae infection ( J:286321 )

• 10 week old (young) mice are permissive to intranasal infection with the EMC 2012 strain of Middle East respiratory syndrome coronavirus (MERS-CoV) , showing a mild but transient weight loss from days 6 to 7 postinoculation (dpi), seroconversion at 35 dpi, virus titers detectable up to 5 dpi in the lungs and eliminated by 7 dpi, and very low copy numbers of viral RNA in the blood at 3 and 5 dpi, indicating that the virus infects and replicates mainly in the lower respiratory tract and is eliminated within 7 days of infection

• 25 week old (adult) mice infected with MERS-CoV show weight loss but recover by 14 dpi, show no obvious signs such as respiratory illness and mortality but show delayed and prolonged inflammatory responses in the lung compared to 10 week old mice

• however, mice infected with MERS-CoV do not show viral RNA in the brain or spleen

homeostasis/metabolism

abnormal cytokine level ( J:286321 )

• MERS-CoV-infected mice (both young and adults) show increased expression of proinflammatory cytokines and chemokines in the lungs, including IP-10, IL-6, IL-13, MCP-1, MIP-1alpha, MIG, IL-12, and IFN-gamma, with higher levels of IP-10, IL-12, and IL-1beta in adult mice and IL-1alpha and IL-17 detected only in the adult mice, and a transient increase in IFN-alpha4 and IFN-beta in adults, indicating a more severe reaction in aged mice

respiratory system

lung inflammation ( J:286321 )

• 10 week old mice infected with MERS-CoV show progressive pulmonary inflammation and develop acute pneumonia from which they recover; inflammatory reactions include partial and/or mild perivascular and peribronchiolar infiltration by mononuclear cells and eosinophils in alveolar areas on 3 and 5 dpi, and severe lung inflammation, including perivascular and alveolar septal thickening causing by infiltrating mononuclear cells on 7 dpi, clearing of inflammatory responses is seen by 35 dpi although focal cellular infiltration is still seen in the peribronchioles and alveolar septa and no inflammation is seen in the brain through 35 dpi

• 25 week old mice infected with MERS-CoV show delayed and prolonged inflammatory responses in the lung compared to 10 week old mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Middle East respiratory syndrome		DOID:0080642		J:286321