Phenotypes for Dnah8 MGI:6473984 MGI Mouse

immotile sperm ( J:297137 )

• diminished sperm movement

• in stage VII-VIII seminiferous tubules, normal round spermatids are seen but elongated tails are not seen and cauda epididymis shows decreased sperm quantity indicating impaired spermatogenesis

abnormal sperm flagellum morphology ( J:297137 )

• sperm show higher rates of abnormal flagella including irregular shapes in some sperm

• 20.5 +/- 12.6% of sperm show irregular flagellum caliber

abnormal outer dense fiber morphology ( J:297137 )

• outer dense fibers are disorganized

abnormal sperm axoneme morphology ( J:297137 )

• the midpiece and principal piece of sperm flagella shows disorganization of microtubules and outer dense fibers

disorganized sperm mitochondrial sheath ( J:297137 )

• midpiece of sperm flagella shows disorganization of mitochondrial sheaths

abnormal sperm fibrous sheath morphology ( J:297137 )

• principal piece of sperm flagella shows disorganization of fibrous sheaths

absent sperm flagellum ( J:297137 )

• 18.5 +/- 17.8% of sperm show absent flagella

coiled sperm flagellum ( J:297137 )

• 25.5% +/- 15.6% of sperm show coiled flagella

kinked sperm flagellum ( J:297137 )

• 9.8% +/- 1.6% of sperm show bent flagella

short sperm flagellum ( J:297137 )

• 25.7 +/- 1.2% of sperm show short flagella

male infertility ( J:297137 )

• males fail to produce offspring

• however, pups are obtained upon intracytoplasmic sperm injection using mutant spermatozoa