Phenotypes for Hsp90b1 Tg(Ddx4-cre)1Dcas MGI:6885740 MGI Mouse

reproductive system phenotype ( J:168319 )

N	• adult males show normal testis weight-to-body weight ratio relative to control males

abnormal sperm midpiece morphology ( J:168319 )

• fluorescent HSPA5 (an ER chaperone) and TO-PRO-3 (DNA) staining revealed strong HSPA5 localization within the abnormal sperm head rather than at the level of the intermediate piece as observed in wild-type sperm

• streptavidin-FITC (biotinylated proteins in mitochondria) and TO-PRO-3 (DNA) staining revealed a stronger streptavidin-FITC labeling concentrated at the abnormal sperm head, unlike in wild-type sperm

abnormal sperm head morphology ( J:168319 )

• all cauda epididymal sperm show head distortions including abnormal heads with (i) a rather elongated head, ii) a rounded-globular head, and iii) an enlarged-deformed head

• 86% of abnormal sperm heads include types (ii) and (iii)

• normal hook-shaped sperm heads are never observed

abnormal acrosome morphology ( J:168319 )

• fluorescent Lens culinaris agglutinin (LCA)-fluorescein isothiocyanate (FITC) (glycoconjugates) and TO-PRO-3 (DNA) staining revealed a diffuse and variable labeling of the acrosome, unlike in wild-type sperm

globozoospermia ( J:168319 )

• ~40% of sperm heads are rounded-globular

enlarged sperm head ( J:168319 )

• ~40% of sperm heads are enlarged and deformed

asthenozoospermia ( J:168319 )

• cauda epididymal sperm are almost immobile

male infertility ( J:168319 )

• adult males are infertile

• however, mating behavior is normal