Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30828
Ang SL, et al., A targeted mouse Otx2 mutation leads to severe defects in gastrulation and formation of axial mesoderm and to deletion of rostral brain. Development. 1996 Jan;122(1):243-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1276058
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Antibody:
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alpha Enhb-1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A:
embryonic day 8.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Otx2tm1Sla/Otx2tm1Sla
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Absent |
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5D
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No expression was observed, indicating that the midbrain and anterior hindbrain regions were missing in the homozygous mutants.
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Analysis Tools
