Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46641
Rhinn M, et al., Sequential roles for Otx2 in visceral endoderm and neuroectoderm for forebrain and midbrain induction and specification. Development. 1998 Mar;125(5):845-56
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1327267
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Antibody:
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alpha Enhb-1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The primary antibody recognizes both En1 and En2 in mouse.
Primary antibody used at a 1:1000 dilution. Seconary antibody used at a 1:200 dilution.
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Results
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Specimen
8C:
embryonic day 8.0
(more  )
Note: 4-somites.
Specimen
8C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somites.
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Specimen
8D:
embryonic day 8.0
(more )
Note: 6-8-somites.
Specimen
8D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-8-somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
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8D
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En is expressed in the mesencephalic-metencephalic region.
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| TS13: future hindbrain |
Present |
Regionally restricted |
8D
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En is expressed in the mesencephalic-metencephalic region.
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Analysis Tools
