Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:47783
Obata Y, et al., Disruption of primary imprinting during oocyte growth leads to the modified expression of imprinted genes during embryogenesis. Development. 1998 Apr;125(8):1553-60
|
Assay type:
|
RT-PCR
|
MGI Accession ID:
|
MGI:1328260
|
Gene symbol:
|
Mest
|
Gene name:
|
mesoderm specific transcript
|
Probe:
|
Mest-pA, Mest-pB
|
Assay notes:
|
Amplification: 30 cycles at 95 C for 30 seconds, 65 C for 30 seconds, and 72 for 60 seconds.
ng/fg PE = parthenogenetic embryo (PE) containing genomes from non-growing (ng) and fully grown oocytes (fg).
fg/fg PE = parthenogenetic embryo containing genomes from two fully grown oocytes.
|
|
Results
|
E9.5
|
TS15: embryo
|
Present
|
1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
|
E9.5
|
TS15: embryo
|
Present
|
1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
|
E9.5
|
TS15: embryo
|
Present
|
1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
|
Treated sample: no data stored |
Treated sample: no data stored |
Treated sample: no data stored |
Treated sample: no data stored |
E12.5
|
TS20: embryo
|
Present
|
1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
|
E12.5
|
TS20: embryo
|
Present
|
1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
|
Treated sample: no data stored |
Treated sample: no data stored |
|