Probe:	mSlu cDNA3
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		2G

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Strong		2I
TS13: neural crest	Present	Regionally restricted	2I	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS13: head somite	Present	Uniform	2I	Transcripts were detected in the whole of the somite.
TS13: trunk somite	Present	Uniform	2I	Transcripts were detected in the whole of the somite.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Strong		2K
TS13: neural crest	Present	Regionally restricted	2K	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS13: head somite	Present	Uniform	2K	Transcripts were detected in the whole of the somite.
TS13: trunk somite	Present	Uniform	2K	Transcripts were detected in the whole of the somite.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3E
TS15: neural crest	Present	Regionally restricted	3E	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS15: trunk mesenchyme	Present	Regionally restricted	3E	mSlu expression was detected exclusively in the lateral mesoderm.
TS15: trunk somite	Present	Regionally restricted	3E	mSlu was detected in sites corresponding to the rostral halves of the somites.
TS15: forelimb bud	Present		3E

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	3G	mSlu was detected in sites corresponding to the rostral halves of the somites.
TS17: trunk somite	Present	Regionally restricted	3G	mSlu was detected in sites corresponding to the rostral halves of the somites.

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present	Regionally restricted	4E	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS13: migrating neural crest	Present		4E
TS13: embryo mesoderm	Absent		4E	Absent from early mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present	Regionally restricted	4G	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS13: migrating neural crest	Present		4G
TS13: embryo mesoderm	Absent		4G	Absent from early mesoderm.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Regionally restricted	4I	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS15: migrating neural crest	Present		4I

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		4M

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	5F	Transcripts were observed in migrating neural crest cells but not in the premigratory neural crest.
TS13: migrating neural crest	Present		5F
TS13: embryo mesoderm	Absent		5F	Absent from early mesoderm.
TS13: primitive streak	Absent		5F

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	6E	mSlu expression was detected exclusively in the lateral mesoderm

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	6G	mSlu expression was detected exclusively in the lateral mesoderm

Structure	Level	Pattern	Image	Note
TS13: head somite	Present	Uniform	7B	Transcripts were detected in the whole of the somite.
TS13: trunk somite	Present	Uniform	7B	Transcripts were detected in the whole of the somite.

Structure	Level	Pattern	Image	Note
TS13: head somite	Present	Uniform	7G	Transcripts were present in the whole of the somite.
TS13: trunk somite	Present	Uniform	7G	Transcripts were present in the whole of the somite.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	7K	mSlu was detected in sites corresponding to the rostral halves of the somites. This corresonded to the neural crest cells migrating across the sclerotome.
TS15: trunk somite	Present	Regionally restricted	7K	mSlu was detected in sites corresponding to the rostral halves of the somites. This corresonded to the neural crest cells migrating across the sclerotome.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		8G
TS17: forelimb bud ectoderm	Present	Regionally restricted	8G	mSlu transcripts were observed in the ventral and dorsal ectoderm of the limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		8H
TS17: forelimb bud ectoderm	Present	Regionally restricted	8H	mSlu transcripts were observed in the ventral and dorsal ectoderm of the limb bud.
TS17: forelimb bud apical ectodermal ridge	Present		8H

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region	Present		8L

Structure	Level	Pattern	Image	Note
TS17: eye	Present	Regionally restricted	9C	Slu transcripts were observed in the lens of the developing eye.