Probe:	mSna cDNA3
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong		2H	mSna expression is particularly strong in the mesoderm as it migrates from the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	2J	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.
TS13: neural crest	Present	Regionally restricted	2J	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	2J	A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	2L	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.
TS13: neural crest	Present	Regionally restricted	2L	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	3F	A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.
TS15: forelimb bud	Present		3F

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		3H

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		4F

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		4H

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	4J	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.
TS15: neural crest	Present	Regionally restricted	4J	Sna was observed in both premigratory and migratory cephalic neural crest cells. Transcripts were expressed in a greater number of migratory crest cells than mSlu.

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme derived from neural crest	Present		4N

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	5E	mSna is present in both the primitive streak and early mesoderm.
TS13: primitive streak	Present	Regionally restricted	5E	mSna is present in both the primitive streak and early mesoderm.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	6F	A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	6H	A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.`

Structure	Level	Pattern	Image	Note
TS13: head somite	Present	Regionally restricted	7D	mSna was confined to cells situated ventrally in the somites, most probably cells undergoing epithelial-to-mesenchyme transition.
TS13: trunk somite	Present	Regionally restricted	7D	mSna was confined to cells situated ventrally in the somites, most probably cells undergoing epithelial-to-mesenchyme transition.

Structure	Level	Pattern	Image	Note
TS13: head somite	Present	Regionally restricted	7H	mSna was confined to cells situated ventrally in the somites, most probably cells undergoing epithelial-to-mesenchyme transition.
TS13: trunk somite	Present	Regionally restricted	7H	mSna was confined to cells situated ventrally in the somites, most probably cells undergoing epithelial-to-mesenchyme transition.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		7L
TS15: neural crest	Present		7L
TS15: sclerotome	Present		7L
TS15: dermatome	Absent

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	8E	mSna transcripts were observed in a more extensive domain of the limb bud mesenchyme than mSlu transcripts.
TS15: forelimb bud ectoderm	Absent		8E

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	8F	mSna transcripts were observed in a more extensive domain of the limb bud mesenchyme than mSlu transcripts.
TS15: forelimb bud ectoderm	Absent		8F

Structure	Level	Pattern	Image	Note
TS21: hand	Present		8K

Structure	Level	Pattern	Image	Note
TS17: eye	Absent		9D

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	10B