Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52599
Mendelsohn C, et al., Stromal cells mediate retinoid-dependent functions essential for renal development. Development. 1999 Mar;126(6):1139-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330386
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Gene symbol:
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Rarb
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Gene name:
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retinoic acid receptor, beta
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Probe:
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mRAR-beta
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Hybridization represents isoform RAR[beta]2 transcripts in the embryonic kidney. Transcript expression is maintained in the knockout mice, although no protein is made.
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Results
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Specimen
Not Shown:
embryonic day 11.0
(more  )
Specimen
Not Shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: metanephros |
Present |
Regionally restricted |
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Expression was detected in a ring of cells at the edge of the metanephric blastema, which may contain presumptive stromal cells.
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Specimen
1C:
embryonic day 12.0
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
Regionally restricted |
1C
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Expression was detected in the renal capsule.
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| TS20: ureteric tip |
Absent |
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1C
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Expression was absent from the ureteric bud.
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| TS20: mesenchyme of metanephros |
Present |
Regionally restricted |
1C
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Expression was detected in presumptive stromal cells surrounding the ureteric bud tips and metanephric mesenchyme and in the presumptive stromal cells surrounding the ureter.
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Specimen
1D:
embryonic day 14.0
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: renal medullary interstitium |
Present |
Regionally restricted |
1D
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Expression was detected in the renal capsule, the subcapsular stromal cells and the cortico-medullary stromal cells.
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Specimen
6A:
embryonic day 14.0
(more )
Note: The plane of section was transverse.
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was transverse.
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Specimen
6B:
embryonic day 14.0; Raratm1Ipc/ Raratm1Ipc, Rarbtm1Ipc/ Rarbtm1Ipc
(more )
Note: The plane of section was transverse. Note that transcripts are generated in knockout mice although the protein is not made.
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Raratm1Ipc/Raratm1Ipc
Rarbtm1Ipc/Rarbtm1Ipc
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was transverse. Note that transcripts are generated in knockout mice although the protein is not made.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: renal medullary interstitium |
Present |
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6B
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Expression was detected in the cortico-medullary stromal cells and the subcapsular stromal cells but was abnormally distributed in a thick ring of cells encircling the mutant kidneys.
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