Gene Expression Data
Assay Details
Assay
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Reference:
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J:53294
Solloway MJ, et al., Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development. 1999 Apr;126(8):1753-68
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333099
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5C left:
embryonic day 9.5
(more )
Specimen
5C left:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
5C left
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Expression is in the dorsal midbrain and along the midbrain/hindbrain boundary.
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TS15: future midbrain |
Present |
Regionally restricted |
5C left
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Expression is in the dorsal midbrain and along the midbrain/hindbrain boundary.
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Specimen
5C right:
(close )
Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Bmp5se/Bmp5se Bmp7tm1Rob/Bmp7tm1Rob
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
5C right
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Expression is in the dorsal midbrain and along the midbrain/hindbrain boundary.
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TS15: future midbrain |
Present |
Regionally restricted |
5C right
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Expression is in the dorsal midbrain and along the midbrain/hindbrain boundary.
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Specimen
5G:
embryonic day 10.5
(more )
Specimen
5G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
5G
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Expression is in the dorsal midbrain, the midbrain/hindbrain isthmus, and the edges of the hindbrain roofplate.
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TS17: midbrain |
Present |
Regionally restricted |
5G
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Expression is in the dorsal midbrain, the midbrain/hindbrain isthmus, and the edges of the hindbrain roofplate.
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Specimen
5H:
(close )
Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Bmp5se/Bmp5se Bmp7tm1Rob/Bmp7tm1Rob
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
5H
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Expression is in the dorsal midbrain, the midbrain/hindbrain isthmus, and the edges of the hindbrain roofplate. The hindbrain roofplate is severely reduced in size in double-mutant embryos.
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TS17: midbrain |
Present |
Regionally restricted |
5H
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Expression is in the dorsal midbrain, the midbrain/hindbrain isthmus, and the edges of the hindbrain roofplate. The hindbrain roofplate is severely reduced in size in double-mutant embryos.
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