Probe:	FGF-3g
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	FGF-3 expression in heterozygous kreisler embryos appears identical to that seen in wild-type controls.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1A	In the developing head, expression is seen within a large portion of the hindbrain.
TS12: embryo ectoderm	Present	Regionally restricted	1A	Expression is seen in the dorsal head ectoderm. In the dorsoventral axis, expression extends from a dorsal-most limit at the junction with the neural crest to a ventral-most limit alongside the foregut.
TS12: future hindbrain neural crest	Absent		1A

Structure	Level	Pattern	Image	Note
TS13: embryo ectoderm	Present	Regionally restricted	1B	The expression domain in the head surface ectoderm includes the developing otic placode and probably some of its immediate neighborhood.
TS13: otic placode	Present		1B
TS13: future hindbrain	Present	Spotted	1B	Expression can be seen to extend from the midbrain-hindbrain junction down to rhombomere 6. The expression in the anterior hindbrain is somewhat diffuse and is clearly at much lower level than rhombomeres 5 and 6, where expression is upregulated.
TS13: future hindbrain posterior to rhombomere 5	Strong		1B	In rhombomeres 5 and 6, expression is upregulated.
TS13: rhombomere 5	Strong		1B	In rhombomeres 5 and 6, expression is upregulated.

Structure	Level	Pattern	Image	Note
TS13: embryo ectoderm	Present	Regionally restricted	1C	The expression domain in the head surface ectoderm includes the developing otic placode and probably some of its immediate neighborhood.
TS13: otic placode	Present	Regionally restricted	1C
TS13: branchial arch	Present	Regionally restricted	1C	Expressed is present in the endoderm lining the ends of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present	Regionally restricted	1D	Expressed is present in the endoderm lining the ends of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Weak	Regionally restricted	1E	When the otic placode has invaginated to form a shallow cup, the expression in this patch of ectoderm has begun to fade, and only the ventral-most portion of the cup still shows expression.
TS14: otic placode	Weak	Regionally restricted	1E	When the otic placode has invaginated to form a shallow cup, the expression in this patch of ectoderm has begun to fade, and only the ventral-most portion of the cup still shows expression.

Structure	Level	Pattern	Image	Note
TS14: ear	Absent			By the 14- to 16-somite stage, no expression in the otic rudiment is visible.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present		1F
TS14: rhombomere 6	Present		1F

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		2A
TS14: branchial arch	Present	Regionally restricted	2A	Expression is evident in the endoderm of the pharyngeal pouches.
TS14: prosencephalon	Present	Regionally restricted	2A	Expression is seen in the extreme rostral forebrain.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present	Regionally restricted	2B	Expression persists until about the 20-somite stage, remaining longest in a stripe in the caudal portion of rhombomere 6.
TS14: rhombomere 6	Present	Regionally restricted	2B	Expression persists until about the 20-somite stage, remaining longest in a stripe in the caudal portion of rhombomere 6.
TS14: 1st branchial pouch endoderm	Present		2B
TS14: 2nd branchial pouch endoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS17: otocyst	Weak	Regionally restricted	2C	Expression is localized to the rostroventrolateral portion of the otocyst.
TS17: ear	Weak	Regionally restricted	2C	There is weak expression in the otic epithelium.
TS17: 1st branchial pouch endoderm	Present	Regionally restricted	2C	Expression is present in the endoderm lining the ends of the pharyngeal pouches.
TS17: 2nd branchial pouch endoderm	Present	Regionally restricted	2C	Expression is present in the endoderm lining the ends of the pharyngeal pouches.
TS17: 3rd branchial pouch endoderm	Present	Regionally restricted	2C	Expression is present in the endoderm lining the ends of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS15: otic pit	Absent			Authors ststes expression detected in otic placode.
TS15: otocyst	Absent			Authors ststes expression detected in otic placode.

Structure	Level	Pattern	Image	Note
TS16: facio-acoustic ganglion complex	Present	Regionally restricted	2D	Expression is present in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted		Expression is present in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Weak	Regionally restricted		Expression in the otic epithelium is significantly reduced, but not entirely lost.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Uniform		Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3A	Expression in this mutant is the same as in wild-type embryos.
TS13: branchial arch	Present	Regionally restricted	3A	Expressed is present in the endoderm lining the ends of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3B	This embryo failed to show the intense expression in the caudal hindbrain, but retained the more diffuse expression in the rostral hindbrain as well as the ectodermal domain of expression in the prospective otic region.
TS13: otic placode	Present	Regionally restricted	3B	This embryo failed to show the intense expression in the caudal hindbrain, but retained the more diffuse expression in the rostral hindbrain as well as the ectodermal domain of expression in the prospective otic region.
TS13: branchial arch	Present	Regionally restricted	3B	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present		3C
TS14: rhombomere 6	Present		3C
TS14: tail bud	Present		3C
TS14: branchial arch	Present	Regionally restricted	3C	Expression is present in the endoderm lining the ends of the pharyngeal pouches.
TS14: prosencephalon	Present	Regionally restricted	3C	Expression is present in the extreme rostral forebrain.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Absent		3D	In all other aspects, expression in this mutant is the same as in wild-type embryos.
TS14: tail bud	Present		3D
TS14: branchial arch	Present	Regionally restricted	3D	Expression in this mutant is the same as in wild-type embryos.
TS14: prosencephalon	Present	Regionally restricted	3D	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	4A	Expression becomes evident in the otic epithelium.
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	4A	Expression could be seen in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	4B	In the mutants there is expression in the otic epithelium at this stage, but the domain is misplaced: instead of lying as usual in the anteroventrolateral wall of the otocyst, it lies in the ventromedial wall.
TS15: facio-acoustic preganglion complex	Absent		4B	No expression could be seen in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	4C	Expression could be seen in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Absent		4D	No expression could be seen in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos even though in the mutants the hindbrain domain is absent.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial pouch endoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.
TS14: 2nd branchial pouch endoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.
TS14: prosencephalon	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial pouch endoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.
TS17: 2nd branchial pouch endoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.
TS17: 3rd branchial pouch endoderm	Present	Regionally restricted		Expression in this mutant is the same as in wild-type embryos.