Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30825
Sporle R, et al., Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development. 1996 Jan;122(1):79-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336673
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Probe:
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Pax1 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4E:
embryonic day 10.5
(more  )
Specimen
4E:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Strong |
Regionally restricted |
4E
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Expression is in the sclerotome in a small anterior stripe and in the posterior half of the somite, extending more dorsally at its posterior margin.
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Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Very strong |
Regionally restricted |
4F
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In mutant embryos, in the thoracic and lumbar regions, expression was much stronger than in wild-type embryos, especially in anterior regions.
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Analysis Tools