Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32016
Daston G, et al., Pax-3 is necessary for migration but not differentiation of limb muscle precursors in the mouse. Development. 1996 Mar;122(3):1017-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1338706
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixation is 3.5% formaldehyde.
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Results
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Specimen
5A:
embryonic day 9.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
5A
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Expression outlines the dermomyotome and extends ventrally past the dorsal margin of the forelimb bud.
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp/Pax3Sp-2H
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
5B
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Expression outlines the dermomyotome. In splotch-mutant embryos, expression does not extend to the dorsal edge of the limb bud.
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Specimen
5H:
embryonic day 9.5
(more )
Note: This section is at the level of the forelimb.
Specimen
5H:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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This section is at the level of the forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
5H
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Expression marks the outline of the dermomyotome.
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Specimen
5I:
embryonic day 9.5; Pax3Sp/ Pax3Sp-2H
(more )
Note: This section is at the level of the forelimb.
Specimen
5I:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp/Pax3Sp-2H
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Note:
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This section is at the level of the forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
5I
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In splotch-mutant embryos, expression does not extend ventrally to the dorsal edge of the limb bud.
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Analysis Tools