Gene Expression Data
Assay Details
Assay
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Reference:
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J:50462
Dassule HR, et al., Analysis of epithelial-mesenchymal interactions in the initial morphogenesis of the mammalian tooth. Dev Biol. 1998 Oct 15;202(2):215-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340492
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Gene symbol:
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Wnt10a
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Gene name:
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wingless-type MMTV integration site family, member 10A
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Results
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Specimen
1A:
embryonic day 11.0
(more )
Specimen
1A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: mandible primordium |
Present |
Regionally restricted |
1G
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Highest levels of expression are mesially and distally with an apparent downregulation in between.
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TS19: tongue |
Absent |
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1G
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Specimen
1M:
embryonic day 12.0
(more )
Specimen
1M:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 12.0
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandible primordium |
Present |
Regionally restricted |
1M
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Expression is completely downregulated in diastema. Expression occurs at midline in incisor domains and further distally in molar primordia. Double in situs of Wnt10a/Shh and Wnt10b/Shh confirm expression in either the same or intermixed cell populations.
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TS20: tongue |
Absent |
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1M
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Specimen
1S:
embryonic day 12.0
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1S:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
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Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandible primordium |
Weak |
Regionally restricted |
1S
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Wnt10a is expressed at the tip of the tooth epithelium.
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Specimen
2A:
embryonic day 13.5
(more )
Note: Bud stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
2A:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
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Bud stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lower jaw molar epithelium |
Strong |
Regionally restricted |
2A
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Wnt10a is strongly expressed at the tip of the invaginating epithelium.
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Specimen
2B:
embryonic day 14.5
(more )
Note: Cap stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
2B:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Cap stage. Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
|
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