Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50462
Dassule HR, et al., Analysis of epithelial-mesenchymal interactions in the initial morphogenesis of the mammalian tooth. Dev Biol. 1998 Oct 15;202(2):215-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340495
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Results
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Specimen
1D:
embryonic day 11.0
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: 1st branchial arch mandibular component |
Strong |
Regionally restricted |
1D
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Shh is expressed at the mesial tip of the jaw in a short, thin strip of epithelial cells that extends distally. Shh is also expressed further distally at the site of amalgamation. Expression was symmetrical on either side of the median sulcus.
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| TS18: tongue |
Present |
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1D
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Specimen
1J:
embryonic day 11.5
(more )
Specimen
1J:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mandible primordium |
Strong |
Regionally restricted |
1J
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The midline expression extends further distally. Highest levels of expression are mesially and distally with an apparent downregulation in between.
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| TS19: tongue |
Present |
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1J
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Specimen
1P:
embryonic day 12.0
(more )
Specimen
1P:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mandible primordium |
Present |
Regionally restricted |
1P
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Expression is completely downregulated in the diastema. Expression occurs at the midline in the incisor domains and further distally in the molar primordia.
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| TS20: tongue |
Present |
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1P
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Specimen
1V:
embryonic day 12.0
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1V:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mandible primordium |
Strong |
Regionally restricted |
1V
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Shh is expressed at the tip of the tooth epithelium.
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Specimen
1Y:
embryonic day 11.0
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1Y:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: tongue |
Present |
Regionally restricted |
1Y
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Shh is expressed in the epithelium. Shh transcripts are also found at the base of the tongue in a V-shaped notch that separates the medial ends of the left and right second pharyngeal arches and in the circumvallate papillae.
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Analysis Tools