Gene Expression Data
Assay Details
Assay
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Reference:
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J:50462
Dassule HR, et al., Analysis of epithelial-mesenchymal interactions in the initial morphogenesis of the mammalian tooth. Dev Biol. 1998 Oct 15;202(2):215-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340545
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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M2-3
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Probe preparation:
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RNA
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Results
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Specimen
1E:
embryonic day 11.0
(more )
Specimen
1E:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1K:
embryonic day 11.5
(more )
Specimen
1K:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: mandible primordium |
Strong |
Regionally restricted |
1K
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Ptc is strongly expressed in the mesenchyme along the mesial-distal axis in a pattern that is complimentary to and somewhat broader than Shh.
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TS19: tongue |
Present |
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1K
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Specimen
1Q:
embryonic day 12.0
(more )
Specimen
1Q:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandible primordium |
Present |
Regionally restricted |
1Q
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Ptc is expressed at high levels immediately beneath the thickened dental epithelium of the incisor. Ptc expression is completely down-regulated in the diastema.
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TS20: tongue |
Present |
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1Q
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Specimen
1W:
embryonic day 12.0
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1W:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 12.0
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandible primordium |
Present |
Regionally restricted |
1W
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As earlier, Ptc is expressed at high levels immediately beneath the thickened involuted dental epithelium of the incisor. Unlike other stages, Ptc is also weakly expressed at the tip of the invaginating epithelial cells.
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Specimen
1Z:
embryonic day 12.5
(more )
Note: Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
Specimen
1Z:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
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Tissue was embedded in 30% BSA, 0.49% gelatin, 20% sucrose in PBS and crosslinked with glutaraldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tongue |
Present |
Regionally restricted |
1Z
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Ptc expression is restricted to the mesenchyme, located immediately beneath Shh-expressing cells.
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