Probe:	mRAR-beta
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	1_RAR-beta
TS20: heart	Absent		1_RAR-beta
TS20: fronto-nasal process mesenchyme	Present	Regionally restricted	1_RAR-beta	Transcripts were regionally restricted to very central frontonasal mesenchymal cells located close to the brain.
TS20: tongue	Present	Regionally restricted	1_RAR-beta	Transcripts were regionally restricted to discrete regions at the base of the tongue.
TS20: mandibular process	Present	Regionally restricted	1_RAR-beta

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process mesenchyme	Strong	Regionally restricted	2A RAR-beta	Transcripts were most abundant in anterior facial mesenchyme [e.g. around the eye and in the frontonasal mesenchyme].
TS17: head mesenchyme	Strong	Regionally restricted	2A RAR-beta	Transcripts were most abundant in anterior facial mesenchyme [e.g. around the eye and in the frontonasal mesenchyme].
TS17: 1st branchial arch maxillary component	Absent		2A RAR-beta
TS17: 1st branchial arch mandibular component	Weak	Regionally restricted	2A RAR-beta	Transcripts were absent from the rostral half of the mandibular arch. There was weak labelling in the caudal half of this arch.
TS17: branchial arch	Trace	Regionally restricted	2A RAR-beta	There was little or no labelling in arches 2 to 6.

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process mesenchyme	Present	Regionally restricted	2B RAR-beta	Transcripts were regionally restricted to very central frontonasal mesenchymal cells located close to the brain.
TS20: tongue	Present	Regionally restricted	2B RAR-beta	Transcripts were regionally restricted to discrete regions at the base of the tongue.
TS20: mandibular process	Present	Regionally restricted	2B RAR-beta
TS20: oral cavity	Present	Regionally restricted	2B RAR-beta	In the oral cavity, transcripts were detected in endoderm-derived epithelium located caudally to the pituitary anlage, while the more rostral ectoderm-derived epithelium was negative.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium mesenchyme	Present		2C RAR-beta

Structure	Level	Pattern	Image	Note
TS22: molar dental lamina	Present	Regionally restricted	3_RAR-beta	Transcripts were detected in the superficial region of the dental lamina, but not in its deeper invagination (i.e., not in the ameloblast layer).
TS22: molar mesenchyme	Present	Regionally restricted	3_RAR-beta	Transcripts were also found in the mesenchymal dental sac that surrounds the tooth germ.

Structure	Level	Pattern	Image	Note
TS20: esophagus epithelium	Strong		4A RAR-beta
TS20: esophagus mesenchyme	Strong	Regionally restricted	4A RAR-beta	Transcripts were detected in the peripheral mesenchyme of the oesophagus.
TS20: heart	Absent		4A RAR-beta
TS20: fronto-nasal process mesenchyme	Present	Regionally restricted	4A RAR-beta	Transcripts were regionally restricted to very central frontonasal mesenchymal cells located close to the brain.
TS20: tongue	Present	Regionally restricted	4A RAR-beta	Transcripts were regionally restricted to discrete regions at the base of the tongue.
TS20: mandibular process	Present	Regionally restricted	4A RAR-beta
TS20: oral cavity	Present	Regionally restricted	4A RAR-beta	In the oral cavity, transcripts were detected in endoderm-derived epithelium located caudally to the pituitary anlage, while the more rostral ectoderm-derived epithelium was negative.
TS20: trachea epithelium	Strong	Regionally restricted	4A RAR-beta	Expression was strong in the endoderm-derived tracheal epithelium.
TS20: trachea mesenchyme	Strong		4A RAR-beta

Structure	Level	Pattern	Image	Note
TS20: lung	Present	Regionally restricted		Transcripts were found only in the epithelium of proximal bronchi, while smaller distal bronchi and the mesodermal components of the lungs were not labelled.

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4B RAR-beta	Expression was detected in the epithelium of the intrapulmonary segmental bronchi, whereas the lung parenchyma, the bronchioles and alveolar ducts were not labelled.

Structure	Level	Pattern	Image	Note
TS20: esophagus epithelium	Strong		4C RAR-beta
TS20: esophagus mesenchyme	Strong	Regionally restricted	4C RAR-beta	Transcripts were detected in the peripheral mesenchyme of the oesophagus.
TS20: stomach	Strong	Regionally restricted	4C RAR-beta	Transcripts were detected in the cardiac portion of the stomach.
TS20: foregut duodenum	Absent		4C RAR-beta
TS20: stomach pyloric region	Absent		4C RAR-beta
TS20: midgut mesenchyme	Weak	Regionally restricted	4C RAR-beta	Expression was weak in the peripheral mesenchyme of the midgut.
TS20: midgut epithelium	Weak		4C RAR-beta
TS20: liver	Absent		4C RAR-beta
TS20: intraembryonic coelom	Present	Regionally restricted	4C RAR-beta	Transcripts were found in the diaphragm.
TS20: pancreas	Present	Regionally restricted	4C RAR-beta	The pancreas showed a cortical, patchy expression.

Structure	Level	Pattern	Image	Note
TS17: gut dorsal mesentery	Present		5A RAR-beta
TS17: mesonephros	Present		5A RAR-beta	Diffuse expression was detected in the mesonephros.
TS17: gonad primordium	Absent		5A RAR-beta	Transcripts were not detected in the cells of the genital ridge.

Structure	Level	Pattern	Image	Note
TS22: renal medullary interstitium	Present		5B RAR-beta
TS22: adrenal gland	Absent		5B RAR-beta
TS22: maturing glomerular tuft	Absent		5B RAR-beta
TS22: medullary renal tubule	Absent		5B RAR-beta

Structure	Level	Pattern	Image	Note
TS20: midgut epithelium	Weak		6A RAR-beta
TS20: midgut mesenchyme	Weak	Regionally restricted	6A RAR-beta	Expression was weak in the peripheral mesenchyme of the midgut.
TS20: genital tubercle	Present	Regionally restricted	6A RAR-beta	Transcripts were restricted to the proximal mesenchyme of the genital tubercle, close to the urogenital sinus, and to the allantoic mesenchyme within the umbilical cord.

Structure	Level	Pattern	Image	Note
TS22: stomach epithelium	Present	Regionally restricted		Expression was in the cardiac region of the stomach.
TS22: esophagus epithelium	Present

Structure	Level	Pattern	Image	Note
TS22: anal canal	Present		6B RAR-beta
TS22: gut mesenchyme	Present	Regionally restricted	6B RAR-beta	Expression was detected in the gut outer mesenchyme layer.
TS22: genital tubercle of male	Present	Regionally restricted	6B RAR-beta	Transcripts were restricted to the central core of the phallus.
TS22: urinary system	Present	Regionally restricted	6B RAR-beta	Transcripts were detected in the urethral epithelium and in the prostatic mesenchyme at the base of the urinary bladder.
TS22: bladder	Weak	Regionally restricted	6B RAR-beta	Transcripts were only weakly expressed in the bladder epithelium.

Structure	Level	Pattern	Image	Note
TS17: eye	Absent
TS17: nasal pit	Absent			Transcripts were undetectable in the ectodermal component of the olfactory pits.
TS17: Rathke's pouch	Present	Regionally restricted		The epithelium of Rathke's pouch was specifically labelled.

Structure	Level	Pattern	Image	Note
TS20: pigmented retinal epithelium	Present		7A RAR-beta
TS20: eye	Present	Regionally restricted	7A RAR-beta	Transcripts were located in the vitreous body and in the condensed mesenchyme around the eye cup which will later form the choroid layer.

Structure	Level	Pattern	Image	Note
TS22: inner ear mesenchyme	Strong		7B RAR-beta
TS22: vestibular component epithelium	Strong	Regionally restricted	7B RAR-beta	Expression was strong in the thickened parts of the vestibular epithelium.

Structure	Level	Pattern	Image	Note
TS21: nose	Present	Regionally restricted	7C RAR-beta	Transcripts were present in the olfactory mesenchyme and in a limited area of the olfactory epithelium.
TS21: olfactory epithelium	Present	Regionally restricted	7C RAR-beta	Transcripts were present in the olfactory mesenchyme and in a limited area of the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS21: nasal cavity respiratory epithelium	Absent
TS21: nose	Present	Regionally restricted		Expression was not detected in the respiratory epithelium and mesenchyme but was present in the olfactory mesenchyme and in a limited area of the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	8A RAR-beta	Transcripts were detected in the apical layer of the epithelium.

Structure	Level	Pattern	Image	Note
TS20: stomach epithelium	Present	Regionally restricted	8C RAR-beta	Transcripts were expressed throughout the thickness of the epithelium in the cardiac region of the stomach.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present		9_RAR-beta

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		10A RAR-beta	Transcripts were detected in cells of the pars distalis anlage and in the adjacent mesenchyme.
TS20: remnant of Rathke's pouch	Weak	Regionally restricted	10A RAR-beta	The dorsal aspect of Rathke's pouch epithelium was very poorly labelled.
TS20: neurohypophysis	Absent		10A RAR-beta

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present	Regionally restricted	10B RAR-beta	Transcripts were located in the pars distalis (in both epithelium and mesenchyme).