Probe:	CRBP I clone1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	1_CRBP
TS20: fronto-nasal process	Trace		1_CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.
TS20: mandibular process	Trace		1_CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong	Regionally restricted	2A CRBP	Transcripts were abundant in central regions of the facial mesenchyme.
TS17: 1st branchial arch	Strong	Regionally restricted	2A CRBP	Transcripts were abundant in the caudal half of the first arch.
TS17: branchial arch	Strong	Regionally restricted	2A CRBP	Transcripts were abundant in more caudal pharyngeal arches.
TS17: 1st branchial arch maxillary component	Trace		2A CRBP
TS17: eye	Strong	Regionally restricted	2A CRBP	Expression was strong throughout the retina and lens.
TS17: lens pit	Strong	Uniform	2A CRBP

Structure	Level	Pattern	Image	Note
TS20: tongue	Present	Uniform	2B CRBP	Transcripts were homogeneously distributed in the tongue (note, however their absence in precartilaginous areas at the base of the tongue).
TS20: fronto-nasal process	Trace		2B CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.
TS20: mandibular process	Trace		2B CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.

Structure	Level	Pattern	Image	Note
TS22: molar mesenchyme	Present	Regionally restricted	3_CRBP	Transcripts were present in the superficial regions of the dental sac.
TS22: molar dental papilla	Present		3_CRBP

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process	Trace		4A CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.
TS20: mandibular process	Trace		4A CRBP	Transcripts were not expressed or at a very low level in the mandible and frontonasal areas.
TS20: trachea mesenchyme	Strong		4A CRBP
TS20: trunk mesenchyme	Strong	Regionally restricted	4A CRBP	Expression was strong in the mesenchyme located between the trachea and the heart.
TS20: trachea epithelium	Strong	Regionally restricted	4A CRBP	Expression in the tracheal epithelium was essentially apical.
TS20: visceral pericardium	Present	Regionally restricted	4A CRBP	Transcripts were specifically detected in the outer or epicardial layer.
TS20: endocardial cushion	Present		4A CRBP
TS20: blood vessel	Present	Regionally restricted	4A CRBP	Transcripts were specifically detected in the wall of large blood vessels close to their junction with cardiac cavities.

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Present
TS20: lung mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		4B CRBP

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Present	Regionally restricted	4C CRBP	CRBP was ubiquitously, but not evenly expressed in the gastrointestinal epithelium and mesenchyme.
TS20: gut mesenchyme	Present	Regionally restricted	4C CRBP	CRBP was ubiquitously, but not evenly expressed in the gastrointestinal epithelium and mesenchyme.
TS20: intraembryonic coelom	Present	Regionally restricted	4C CRBP	The diaphragm was homogeneously labelled.
TS20: liver	Present	Uniform	4C CRBP
TS20: pancreas	Present	Uniform	4C CRBP

Structure	Level	Pattern	Image	Note
TS17: gut dorsal mesentery	Present		5A CRBP
TS17: mesonephros	Present		5A CRBP
TS17: mesonephric tubule	Strong		5A CRBP

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		5B CRBP
TS22: maturing glomerular tuft	Strong		5B CRBP
TS22: adrenal gland	Absent		5B CRBP

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Present	Regionally restricted	6A CRBP	CRBP was ubiquitously, but not evenly expressed in the gastrointestinal epithelium and mesenchyme.
TS20: gut mesenchyme	Present	Regionally restricted	6A CRBP	CRBP was ubiquitously, but not evenly expressed in the gastrointestinal epithelium and mesenchyme.
TS20: genital tubercle	Present	Regionally restricted	6A CRBP	Transcripts were abundant in the mesenchyme around the urogenital sinus, decreasing towards the distal tip of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle of male	Weak	Regionally restricted	6B CRBP	Transcripts were very poorly expressed in the central core of the phallus.
TS22: gut mesenchyme	Present	Regionally restricted	6B CRBP	Expression was detected in the gut outer mesenchyme layer.
TS22: gut epithelium	Trace		6B CRBP	There was no or weak expression in the gut epithelium.

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	7A CRBP	Expression was present in the neural retina, with particularly strong signals in the superficial layer.
TS20: pigmented retinal epithelium	Present		7A CRBP

Structure	Level	Pattern	Image	Note
TS22: inner ear mesenchyme	Strong		7B CRBP
TS22: inner ear epithelium	Strong	Regionally restricted	7B CRBP	Transcripts were abundant in most of the inner ear epithelium.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present	Regionally restricted		Transcripts were uniformly expressed in the epithelial and mesenchymal layers of the olfactory pits.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Strong	Regionally restricted	7C CRBP	Transcripts were strongly expressed in the mesenchyme surrounding the nasal cavities.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Strong	Regionally restricted	8A CRBP	Transcripts were strongly expressed in the mesenchyme surrounding the nasal cavities.

Structure	Level	Pattern	Image	Note
TS20: stomach epithelium	Present	Regionally restricted	8C CRBP	Expression was highest at the apical surface within the epithelium of the cardiac portion of the stomach.

Structure	Level	Pattern	Image	Note
TS15: limb	Absent

Structure	Level	Pattern	Image	Note
TS17: limb	Absent

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	9_CRBP	Transcripts were detected only in the mesenchyme of the distal extremities of the limbs, mainly in the interdigital mesenchyme.
TS21: hindlimb interdigital region mesenchyme	Present	Regionally restricted	9_CRBP	Transcripts were detected only in the mesenchyme of the distal extremities of the limbs, mainly in the interdigital mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted		The epithelium of Rathke's pouch was specifically labelled.

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		10A CRBP
TS20: adenohypophysis pars intermedia	Present		10A CRBP
TS20: neurohypophysis	Absent		10A CRBP

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present	Regionally restricted	10B CRBP	Transcripts were observed in the mesenchymal, but not epithelial, tissues of the whole pituitary gland.