Results
|
Specimen
2A:
embryonic day 8.25
(more )
Specimen
2A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2B:
embryonic day 9.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: heart |
Present |
|
2B
|
|
TS15: trunk somite |
Present |
|
2B
|
|
TS15: extraembryonic component |
Present |
Regionally restricted |
2B
|
CD44 is expressed in the amnion and allantois.
|
TS15: branchial arch |
Strong |
Regionally restricted |
2B
|
Strong staining is observed in the branchial clefts.
|
TS15: embryo |
Strong |
Regionally restricted |
2B
|
Strong staining is observed in a population of cells dispersed through the embryo.
|
TS15: forelimb bud ectoderm |
Strong |
Regionally restricted |
2B
|
At limb bud stage 1-2, CD44 protein is localized to the ectoderm on the ventral side of the limb bud. CD44 expression is not detected on the dorsal side.
|
TS15: embryo mesenchyme |
Present |
Scattered |
2B
|
A large number of positive cells arise in the mesenchyme of the embryo, appearing as a scattered population first in the head region and then throughout the embryo.
|
Specimen
2C:
embryonic day 9.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: endocardial lining |
Weak |
|
2C
|
|
TS15: cardiac muscle tissue |
Present |
|
2C
|
Expression is abundant in the developing myocardium.
|
TS15: arterial system |
Strong |
Regionally restricted |
2C
|
The arterial system displays high CD44 protein expression. For example, CD44 protein is detected in the endothelial angioblasts lining the wall of the dorsal aortae.
|
TS15: dorsal aorta |
Strong |
Regionally restricted |
2C
|
The arterial system displays high CD44 protein expression. For example, CD44 protein is detected in the endothelial angioblasts lining the wall of the dorsal aortae.
|
TS15: venous system |
Absent |
|
2C
|
|
TS15: embryo mesenchyme |
Present |
Regionally restricted |
2C
|
A proportion of the CD44-positive cells can be seen in pathways suggestive of migratory neural crest.
|
TS15: migrating neural crest |
Present |
Regionally restricted |
2C
|
A proportion of the CD44-positive cells can be seen in pathways suggestive of migratory neural crest.
|
Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A:
embryonic day 8.0
(more )
Note: 1-2 somites.
Specimen
3A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
1-2 somites.
|
Specimen
3B:
embryonic day 8.25
(more )
Note: 5-6 somites.
Specimen
3B:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
5-6 somites.
|
Specimen
3C:
embryonic day 8.5
(more )
Note: 7-8 somites.
Specimen
3C:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
7-8 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: trunk somite |
Present |
Graded |
3C
|
The highest levels of expression are seen in the presomitic mesoderm and in the most posterior somites, with the levels of expression diminishing towards the anterior of the embryo and the most mature somites no longer expressing CD44.
|
TS13: trunk unsegmented mesenchyme |
Present |
Graded |
3C
|
The highest levels of expression are seen in the presomitic mesoderm and in the most posterior somites, with the levels of expression diminishing towards the anterior of the embryo and the most mature somites no longer expressing CD44.
|
Specimen
3D:
embryonic day 8.75
(more )
Specimen
3D:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: trunk somite |
Present |
Regionally restricted |
3D
|
During maturation, the protein associates with the sclerotome in the central regions of the positive somites and the outer somitic regions are CD44-negative.
|
Specimen
3E:
embryonic day 10.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: arterial system |
Strong |
|
3E
|
The arterial system displays high CD44 protein expression. For example, CD44 protein is detected in the intersomitic arteries.
|
Specimen
3F:
embryonic day 10.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: notochord |
Strong |
Regionally restricted |
3F
|
The notochord in the tail expresses high levels of CD44 protein.
|
TS17: tail |
Present |
Regionally restricted |
3F
|
Expression was detected in a patch of cells on the ventral aspect of the tailbud.
|
TS17: tail mesenchyme |
Present |
Scattered |
3F
|
A large number of positive cells arise in the mesenchyme of the embryo, appearing as a scattered population throughout the embryo including the tail.
|
Specimen
3G:
embryonic day 10.5
(more )
Specimen
3G:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: notochord |
Strong |
Regionally restricted |
3G
|
The notochord in the tail expresses high levels of CD44 protein.
|
Specimen
4A:
embryonic day 9.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud ectoderm |
Strong |
Regionally restricted |
4A
|
At limb bud stage 1-2, CD44 protein is localized to the ectoderm on the ventral side of the limb bud. CD44 expression is not detected on the dorsal side.
|
Specimen
4B:
embryonic day 9.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud ectoderm |
Strong |
Regionally restricted |
4B
|
At limb bud stage 1-2, CD44 protein is localized to the ectoderm on the ventral side of the limb bud. CD44 expression is not detected on the dorsal side.
|
Specimen
4C:
embryonic day 10.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4D:
embryonic day 10.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4E:
embryonic day 10.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4F:
embryonic day 12.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb |
Present |
Regionally restricted |
4F
|
At limb bud stage 8, expression in the AER is maintained, but CD44 is also expressed in the condensing limb-bud mesenchyme prior to the cartilage-generating chondrogenic process.
|
Specimen
not shown:
embryonic day 8.5
(more )
Note: 7-8 somites.
Specimen
not shown:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
7-8 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: 1st branchial pouch endoderm |
Present |
Regionally restricted |
|
CD44 protein first appears in the branchial arch region as a patch in the endoderm of the developing first pharyngeal pouch adjacent to the developing first branchial arch.
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: arterial system |
Strong |
Regionally restricted |
5A
|
The arterial system displays high CD44 protein expression. For example, CD44 protein is detected in the endothelial angioblasts lining the wall of the dorsal aortae.
|
TS15: dorsal aorta |
Strong |
Regionally restricted |
5A
|
The arterial system displays high CD44 protein expression. For example, CD44 protein is detected in the endothelial angioblasts lining the wall of the dorsal aortae.
|
TS15: 1st branchial groove |
Present |
|
5A
|
|
TS15: 2nd branchial groove |
Present |
|
5A
|
|
Specimen
5D, 5E:
embryonic day 9.5
(more )
Specimen
5D, 5E:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5F, 5G:
embryonic day 10.5
(more )
Specimen
5F, 5G:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5H & 5I:
embryonic day 10.5
(more )
Specimen
5H & 5I:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6A:
embryonic day 10.75
(more )
Specimen
6A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.75
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: naso-lacrimal groove |
Present |
Regionally restricted |
6A
|
CD44 is expressed in the ectoderm of the nasolacrimal groove.
|
TS17: heart |
Present |
|
6A
|
|
TS17: embryo mesenchyme |
Present |
Scattered |
6A
|
A large number of positive cells arise in the mesenchyme of the embryo, appearing as a scattered population throughout the embryo including the limb buds and tail.
|
Specimen
6C:
embryonic day 10.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: branchial arch artery |
Strong |
Regionally restricted |
6C
|
The arterial system displays high CD44 protein expression. CD44 is observed in the cells lining the branchial arch arteries.
|
TS17: nasal pit |
Present |
Regionally restricted |
6C
|
CD44 is expressed in the posterolateral rim of the developing nasal pit.
|
Specimen
6D:
embryonic day 10.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7A:
embryonic day 9.5
(more )
Specimen
7A:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7B:
embryonic day 10.5
(more )
Specimen
7B:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7C:
embryonic day 10.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7D & 7E:
embryonic day 10.5
(more )
Specimen
7D & 7E:
(close )
Genetic Background:
|
TO
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|