Probe:	Crabp1 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: rhombomere 2	Strong		4C
TS13: rhombomere 4	Strong		4C
TS13: rhombomere 3	Absent		4C
TS13: future hindbrain	Present	Regionally restricted	4C	Strong expression in prospective rhombomere 6. Expression in prospective rhombomere 7.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 2	Present	Regionally restricted	4D	The area of expression is larger than in wild-type embryos.
TS13: rhombomere 3	Absent		4D	The area is not sharply defined and extends caudally within the myelencephalon.
TS13: future hindbrain	Present	Regionally restricted	4D	Posteriorly (prospective rhombomere 7) expression is similar to wild-type embryos.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch mesenchyme derived from neural crest	Strong		5E
TS15: 3rd branchial arch mesenchyme derived from neural crest	Strong		5E
TS15: head mesenchyme derived from neural crest	Strong	Regionally restricted	5E	Expression in the cells accumulating in branchial arch 4. Expression in the frontonasal region in cells derived from the forebrain and midbrain.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted	5F	Labelling in the frontonasal region of cells derived from the forebrain and midbrain is similar to that of wild-type embryos.
TS15: future hindbrain	Present	Regionally restricted	5F	Labelling is in a few neural crest cells in the myelencephalon that appear to have taken abberant migratory routes.