Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56581
Daubas P, et al., Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development. 2000 Jan;127(2):319-31
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1352729
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
8. D.:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more  )
Note: Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
Specimen
8. D.:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb |
Present |
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8D
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| TS17: notochord |
Present |
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8D
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| TS17: brain |
Present |
Regionally restricted |
8D
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Expression is detected in the brain in the longitudinal zone along the entire CNS and ends anteriorly.
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Specimen
8. E.:
embryonic day 10.5
(more )
Specimen
8. E.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
Regionally restricted |
8E
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Expression is detected in the brain in the longitudinal zone along the entire CNS and ends anteriorly.
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| TS17: diencephalon |
Present |
Regionally restricted |
8E
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Expression is present in ventral prosomere 4.
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| TS17: midbrain |
Present |
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8E
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Specimen
8. F.:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
Specimen
8. F.:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain ventricular layer |
Strong |
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8F
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Expression is detected in the ventral mesencephalon; it is strong in the ventricular zone of the neuroepithelium and its pattern crosses the midline.
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| TS17: diencephalon lateral wall ventricular layer |
Strong |
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8F
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Expression is detected in the caudal diencephalon; it is strong in the ventricular zone of the neuroepithelium and its pattern crosses the midline.
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Specimen
E12.5:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
Specimen
E12.5:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Shh; salmon - Myf5 (assay MGI:5433581).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
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Expression is present in the mesencephalon-prosomere 1 region.
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