Gene Expression Data
Assay Details
Assay
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Reference:
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J:62028
Charite J, et al., The bHLH transcription factor dHAND controls Sonic hedgehog expression and establishment of the zone of polarizing activity during limb development. Development. 2000 Jun;127(11):2461-70
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1858472
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1. D.:
embryonic day 9.5
(more )
Specimen
1. D.:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1. E.:
embryonic day 9.75
(more )
Specimen
1. E.:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Weak |
Regionally restricted |
1E
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Expression is detected in the posterior margin of the right forelimb.
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Specimen
1. J.:
embryonic day 10.75
(more )
Specimen
1. J.:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.75
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: forelimb bud |
Present |
Regionally restricted |
1J
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Expression is detected in the posterior margin of the right forelimb.
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Specimen
1. L.:
embryonic day 10.75
(more )
Specimen
1. L.:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.75
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
1. N.:
embryonic day 11.5
(more )
Specimen
1. N.:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
6. A.:
embryonic day 10.5
(more )
Specimen
6. A.:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
6A
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Expression is detected in the posterior margin of the right forelimb.
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Specimen
2. B. WT:
embryonic day 10.0
(more )
Specimen
2. B. WT:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.0
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Regionally restricted |
2B WT
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Expression is detected in the posterior margin of forelimb.
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Specimen
2. B. -/-:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Hand2tm1Dsr/Hand2tm1Dsr
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2. C.:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Hand2tm1Dsr/Hand2tm1Dsr
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Age:
|
embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: embryo |
Present |
Regionally restricted |
2C
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Axial expression is detected.
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