Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:64329
Welsh IC, et al., Loss of late primitive streak mesoderm and interruption of left-right morphogenesis in the Ednrb(s-1Acrg) mutant mouse. Dev Biol. 2000 Sep 1;225(1):151-68
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1891726
|
|
Gene symbol:
|
Lefty1
|
|
Gene name:
|
left right determination factor 1
|
|
Probe:
|
Lefty probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4E wt:
embryonic day 8.0
(more  )
Note: Embryo is at the 4-5 somite stage.
Specimen
4E wt:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 4-5 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
4E WT
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
| TS12: neural plate |
Present |
Regionally restricted |
4E WT
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
Specimen
4E -/-:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Ednrbs-1Acrg/Ednrbs-1Acrg
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 4-5 somite stage.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Ednrbs-1Acrg/Ednrbs-1Acrg
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 3 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
| TS12: neural plate |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: Embryo is at the 3-4 somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 3-4 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
| TS12: neural plate |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: Embryo is at the 5-6 somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 5-6 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm. expression is also slightly higher then the 3-4 somite stage.
|
| TS12: neural plate |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm. expression is also slightly higher then the 3-4 somite stage.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Ednrbs-1Acrg/Ednrbs-1Acrg
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 4 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm and is at a reduced level compared to wildtype.
|
| TS12: neural plate |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm and is at a reduced level compared to wildtype.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Ednrbs-1Acrg/Ednrbs-1Acrg
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Embryo is at the 5-6 somite stage.
|
|
Analysis Tools