Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64329
Welsh IC, et al., Loss of late primitive streak mesoderm and interruption of left-right morphogenesis in the Ednrb(s-1Acrg) mutant mouse. Dev Biol. 2000 Sep 1;225(1):151-68
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1891727
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4E wt:
embryonic day 8.0
(more  )
Note: Embryo is at the 4-5 somite stage.
Specimen
4E wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 4-5 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
4E WT
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
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| TS12: neural plate |
Present |
Regionally restricted |
4E WT
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
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Specimen
4E -/-:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 4-5 somite stage.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 3 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
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| TS12: neural plate |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
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Specimen
not shown:
embryonic day 8.0
(more )
Note: Embryo is at the 3-4 somite stage.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 3-4 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
|
| TS12: neural plate |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm.
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Specimen
not shown:
embryonic day 8.0
(more )
Note: Embryo is at the 5-6 somite stage.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 5-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm at a slightly higher level then the 3-4 somite stage.
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| TS12: neural plate |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm at a slightly higher level then the 3-4 somite stage.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm but at a reduced level compared to wildtype.
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| TS12: neural plate |
Present |
Regionally restricted |
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Expression is detected on the left side of the prospective floor plate and the lateral plate mesoderm but at a reduced level compared to wildtype.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 5-6 somite stage.
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Analysis Tools