Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32030
Graham A, et al., The murine Hox-2 genes display dynamic dorsoventral patterns of expression during central nervous system development. Development. 1991 May;112(1):255-64
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1929177
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Gene symbol:
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Hoxb3
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Gene name:
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homeobox B3
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Probe:
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Hoxb3 probe4
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1 p:
embryonic day 12.5
(more  )
Specimen
1 p:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Strong |
Regionally restricted |
1p
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Expression in a 'M'-shaped pattern with strong dorsal/lateral labeling.
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Specimen
1 q:
embryonic day 12.5
(more )
Specimen
1 q:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Strong |
Regionally restricted |
1q
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Expression in a 'M'-shaped pattern with strong dorsal/lateral labeling.
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Specimen
1 r:
embryonic day 12.5
(more )
Specimen
1 r:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Strong |
Regionally restricted |
1r
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A strong dorsal domain of expression with a sharp restriction.
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| TS20: spinal cord ventricular layer |
Weak |
Regionally restricted |
1r
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A low or undetectable level of expression in the ventricular layer.
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Specimen
1 s:
embryonic day 12.5
(more )
Specimen
1 s:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Strong |
Regionally restricted |
1s
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A strong dorsal domain of expression with a sharp restriction
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| TS20: spinal cord ventricular layer |
Weak |
Regionally restricted |
1s
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A low or undetectable level of expression in the ventricular layer.
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Specimen
1 t:
embryonic day 12.5
(more )
Specimen
1 t:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2 g:
embryonic day 10.5
(more )
Specimen
2 g:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2 i:
embryonic day 10.5
(more )
Specimen
2 i:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
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2i
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| TS17: neural tube |
Present |
Uniform |
2i
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Uniform expression across the neural tube. Uniform expression across the neural tube. Uniform expression across the neural tube.
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Specimen
3 f:
embryonic day 11.5
(more )
Specimen
3 f:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Strong |
Regionally restricted |
3f
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High levels in the lateral regions and no expression in the ventral motor horns.
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Specimen
not shown:
embryonic day 9.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future spinal cord neural fold |
Present |
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Uniform expression across transverse sections of the neural tube and in the closing neural folds.
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| TS14: neural tube |
Present |
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Uniform expression across transverse sections of the neural tube and in the closing neural folds.
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Analysis Tools