Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39204
Lee SM, et al., Evidence that FGF8 signalling from the midbrain-hindbrain junction regulates growth and polarity in the developing midbrain. Development. 1997 Mar;124(5):959-69
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1930309
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 probe15
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 8.0
(more  )
Note: 6 somites.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
1A
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Expression is in the mesencephalon-metencephalon region.
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| TS12: future midbrain |
Present |
Regionally restricted |
1A
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Expression is in the mesencephalon-metencephalon region.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt1tm1Brd/Wnt1tm1Brd
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
1B
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Expression is in the mesencephalon-metencephalon region.
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| TS12: future midbrain |
Present |
Regionally restricted |
1B
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Expression is in the mesencephalon-metencephalon region.
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Specimen
1C:
embryonic day 8.5
(more )
Note: 9 somites.
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somites.
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Specimen
1D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt1tm1Brd/Wnt1tm1Brd
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
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1D
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Expression is reduced in the metencephalon. Authors also state that mesencepahlon development is reduced at this time.
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Specimen
1E:
embryonic day 9.0
(more )
Note: 15 somites.
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
1E
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Expression was restricted to a narrow band immediately posterior to the isthmus.
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt1tm1Brd/Wnt1tm1Brd
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somites.
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Specimen
6A:
embryonic day 13.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindbrain |
Present |
Regionally restricted |
6A
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Expression is in the mesencephalon-metencephalon region.
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| TS21: midbrain |
Present |
Regionally restricted |
6A
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Expression is in the mesencephalon-metencephalon region.
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Analysis Tools