Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:21617
Gunther T, et al., Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development. 1994 Nov;120(11):3119-30
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1933416
|
|
Gene symbol:
|
Pax3
|
|
Gene name:
|
paired box 3
|
|
Probe:
|
Pax3 probe2
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
4E:
embryonic day 12.5
(more  )
Specimen
4E:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4E
|
Expression was restricted to proliferating neurons in the ventricular layer of the alar plate.
|
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
4E
|
Expression was restricted to proliferating neurons in the ventricular layer of the alar plate.
|
Specimen
4F:
embryonic day 12.5; Rab23opb/ Rab23opb
(more )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
4F:
(close )
|
Genetic Background:
|
involves: C57BL/6 * NMRI
|
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4F
|
In severely affected spinal cord regions, no expression was evident in a region of cells in the dorsalmost part.
|
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
4F
|
In severely affected spinal cord regions, no expression was evident in a region of cells in the dorsalmost part.
|
|
Analysis Tools
