Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:11854
Ontell M, et al., Contractile protein gene expression in primary myotubes of embryonic mouse hindlimb muscles. Development. 1993 Apr;117(4):1435-44
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2135991
|
Gene symbol:
|
Actc1
|
Gene name:
|
actin, alpha, cardiac muscle 1
|
Probe:
|
alpha-cardiac actin
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1D:
embryonic day 11.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud |
Present |
|
1D
|
Expression is detected in the hindlimb muscles and somites.
|
TS19: trunk somite |
Present |
|
1D
|
Expression is detected in the hindlimb muscles and somites.
|
Specimen
4C:
embryonic day 12.0
(more )
Specimen
4C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb |
Present |
Regionally restricted |
4C
|
Expression is detected in the hindlimb muscles and somites.
|
Specimen
5B:
embryonic day 13.0
(more )
Specimen
5B:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hindlimb |
Present |
Regionally restricted |
5B
|
|
Specimen
6B:
embryonic day 14.0
(more )
Specimen
6B:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindlimb |
Present |
Regionally restricted |
6B
|
Expression is detected in the soleus and extensor digitorum longus muscles.
|
Specimen
E10:
embryonic day 10.0
(more )
Note: Temporal expression diagrammed in Fig. 2
Specimen
E10:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Temporal expression diagrammed in Fig. 2
|
Specimen
E11:
embryonic day 11.0
(more )
Note: Temporal expression diagrammed in Fig. 2
Specimen
E11:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Temporal expression diagrammed in Fig. 2
|
Specimen
E12.5:
embryonic day 12.5
(more )
Note: Temporal expression diagrammed in Fig. 2
Specimen
E12.5:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Temporal expression diagrammed in Fig. 2
|
Specimen
1K:
embryonic day 11.5
(more )
Specimen
1K:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud |
Present |
Regionally restricted |
1K
|
Expression is detected in the hindlimb muscles and somites.
|
TS19: trunk somite |
Present |
Regionally restricted |
1K
|
Expression is detected in the hindlimb muscles and somites.
|
|