Probe:	Myf5 probe2
Probe preparation:	Antisense labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		4A
TS17: myotome	Present		4A	Expression in the myotomes of all somites.
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	4A	Expression in the hypoglossal stream.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		4B	Expression in the limbs was severely reduced in the mutants compared to control embryos.
TS17: myotome	Present		4B	Expression in the myotomes of all somites.
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	4B	Expression in the hypoglossal stream.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4C	Expression in differentiating cells in the dorsal and ventral forelimb.
TS17: trunk mesenchyme	Present	Regionally restricted	4C	Expression in the lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4D	Expression only ventroproximally in the anterior half of the forelimb.
TS17: trunk mesenchyme	Present	Regionally restricted	4D	Expression in the lateral plate mesoderm. The density of expressing cells is higher in the mutants than in the controls.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	5A	The endpoint of the hypoglossal stream in the floor of the first arch where the tongue anlage is located contains expressing cells.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	5B	The endpoint of the hypoglossal stream in the floor of the first arch where the tongue anlage is located contains expressing cells, which are reduced in number in mutant embryos compared to control embryos.