Probe:	Wt1 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS14: intraembryonic coelom	Present	Regionally restricted	1a	Expression detected in the lateral lining of the coelomic cavity.

Structure	Level	Pattern	Image	Note
TS15: intraembryonic coelom	Present	Regionally restricted	1b	Expression detected over the epithelium bounding the coelomic cavity with maximal expression at the lateral (outer) edge.
TS15: gut	Present	Regionally restricted	1b	Expression is present in the mesothelium of the heart and gut.
TS15: heart	Present	Regionally restricted	1b	Expression is present in the mesothelium of the heart and gut.
TS15: embryo mesenchyme	Present	Regionally restricted	1b	Expression detected in two small areas of mesenchyme on either side of the caudal boundary of the coelomic cavity.
TS15: nephric duct	Absent		1b
TS15: genitourinary system	Present	Regionally restricted	1b	Expression is present over the urogenital ridge, particularly the mesonephros.
TS15: mesonephros	Present	Regionally restricted	1b	Expression is present over the urogenital ridge, particularly the mesonephros.

Structure	Level	Pattern	Image	Note
TS16: genitourinary system	Present		2a/b
TS16: mesonephros	Present		2a/b
TS16: embryo mesenchyme	Present	Regionally restricted	2a/b	Expression detected in some of the mesenchyme adjacent to the urogenital ridge and the mesothelial linings of the intracoelomic organs.
TS16: gut	Present	Regionally restricted	2a/b	Expression detected in some of the mesenchyme adjacent to the urogenital ridge and the mesothelial linings of the intracoelomic organs.
TS16: heart	Present	Regionally restricted	2a/b	Expression detected in some of the mesenchyme adjacent to the urogenital ridge and the mesothelial linings of the intracoelomic organs.
TS16: liver	Present	Regionally restricted	2a/b	Expression detected in some of the mesenchyme adjacent to the urogenital ridge and the mesothelial linings of the intracoelomic organs.

Structure	Level	Pattern	Image	Note
TS18: ureteric bud	Absent		3a/b
TS18: metanephric mesenchyme	Weak		3a/b	Expression detected in the metanephric blastula.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Strong	Regionally restricted	3c/d	Expression is detected in the condensed mesenchyme and blastemal cells of the metanephros and the genital ridge.
TS20: mesenchyme of metanephros	Strong	Regionally restricted	3c/d	Expression is detected in the condensed mesenchyme and blastemal cells of the metanephros and the genital ridge.
TS20: nephrogenic interstitium	Strong	Regionally restricted	3c/d	Expression is detected in the condensed mesenchyme and blastemal cells of the metanephros and the genital ridge.
TS20: urinary system	Strong	Regionally restricted	3c/d	Expression is detected in the condensed mesenchyme and blastemal cells of the metanephros and the genital ridge.
TS20: nephric duct	Absent		3c/d	Expression is absent in the ureteric bud and the mesonephric duct.
TS20: ureter	Absent		3c/d	Expression is absent in the ureteric bud and the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS23: renal cortex	Strong	Regionally restricted	3e	Expression is detected over the outer cortex of stem cells.
TS23: early nephron	Strong		3e
TS23: ureter	Absent		3e

Structure	Level	Pattern	Image	Note
TS26: glomerular tuft	Strong		3g/h
TS26: nephrogenic interstitium	Weak		3g/h

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Regionally restricted	3i	Expression is restricted to the podocyte layer of the glomeruli.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Weak	Regionally restricted	4a/b	Weak patches of expression are detected in a narrow linear domain in the central region of the spinal cord at the boundary of the mantle and ependymal layers.
TS18: neural tube mantle layer	Weak	Regionally restricted	4a/b	Weak patches of expression are detected in a narrow linear domain in the central region of the spinal cord at the boundary of the mantle and ependymal layers.
TS18: neural tube ventricular layer	Weak	Regionally restricted	4a/b	Weak patches of expression are detected in a narrow linear domain in the central region of the spinal cord at the boundary of the mantle and ependymal layers.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	4c/d	Expression is detected in a small domain in the spinal cord at the border of the ependymal amd mantle layers. The outer ependymal layer is unlabelled and labelling is stronger then at E11.
TS20: spinal cord ventricular layer	Present	Regionally restricted	4c/d	Expression is detected in a small domain in the spinal cord at the border of the ependymal amd mantle layers. The outer ependymal layer is unlabelled and labelling is stronger then at E11.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present	Regionally restricted	4e/f	Expression has moved ventrally toward the floor plate.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present		4g/h	Expression is detected in the ventral horn of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4i/j	Expression is detected in the spinal cord between the mantle and ependymal layers and well away from the mantle. This sagittal section shows expression is uniform along the length of the spinal cord.
TS20: spinal cord mantle layer	Present	Regionally restricted	4i/j	Expression is detected in the spinal cord between the mantle and ependymal layers and well away from the mantle. This sagittal section shows expression is uniform along the length of the spinal cord.
TS20: spinal cord ventricular layer	Present	Regionally restricted	4i/j	Expression is detected in the spinal cord between the mantle and ependymal layers and well away from the mantle. This sagittal section shows expression is uniform along the length of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: gonad	Strong	Regionally restricted	5a/b	Expression is strong in the lining of the gonads. In both sexes the developing sex cords express the gene, but in males neither the intervening loose mesenchyme nor the germ cells show evidence of labelling.
TS21: reproductive system	Present	Patchy	5a/b	Expression is patchy in interstitial region.
TS21: nephric duct	Absent		5a/b	Expression is absent in the wolffian and mullerian ducts and the mesonephric tubule.
TS21: paramesonephric duct	Absent		5a/b	Expression is absent in the wolffian and mullerian ducts and the mesonephric tubule.

Structure	Level	Pattern	Image	Note
TS21: anterior abdominal wall muscle	Present	Regionally restricted	5c/d	Expression is detected in the differentiating body wall musculature.
TS21: body wall mesenchyme	Present	Regionally restricted	5c/d	Expression is detected in the differentiating body wall musculature.
TS21: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	5c/d	Expression is detected in the differentiating body wall musculature.

Structure	Level	Pattern	Image	Note
TS23: 4th ventricle	Present	Regionally restricted	5e	Expression is restricted to a small region of the in the rrof of the 4th ventricle thatw as limited to the diverticulum of the ependymal layer close to the rostral portion of the medulla oblongata. Lower magnification was shown in the inset.
TS23: medulla oblongata	Present	Regionally restricted	5e	Expression is restricted to a small region of the in the rrof of the 4th ventricle thatw as limited to the diverticulum of the ependymal layer close to the rostral portion of the medulla oblongata. Lower magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS23: early nephron	Strong		5g/h
TS23: bladder	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: hindgut	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: mesorchium	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: metanephros	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: midgut	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: pancreas	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: spleen primordium	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: testis	Strong	Regionally restricted	5g/h	Expression is detected in the mesothelial lining.
TS23: ureter	Absent		5g/h
TS23: renal cortex	Strong	Regionally restricted	5g/h	Expression is detected over the outer cortex of stem cells.

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6a/b	Expression is detected in the mesothelial lining and sex-cords.

Structure	Level	Pattern	Image	Note
TS16: mesonephros	Present	Regionally restricted	2c	Expression is detected in the early mesonephros vesicle and later glomerular structures.
TS16: mesonephric tubule	Absent		2c	Expression was not detected over the remainder of the mesonephric tubules or the mesonephric duct.
TS16: nephric duct	Absent		2c	Expression was not detected over the remainder of the mesonephric tubules or the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent
TS12: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS18: metanephric mesenchyme	Strong
TS18: gonad primordium	Present
TS18: mesonephros	Present
TS18: greater sac mesothelium	Present	Regionally restricted		Expression is detected in the epithelium bounding the coelom, particularly over the (outer) parietal lining, and the mesothelium of the heart and gut.
TS18: intraembryonic coelom	Present	Regionally restricted		Expression is detected in the epithelium bounding the coelom, particularly over the (outer) parietal lining, and the mesothelium of the heart and gut.
TS18: omental bursa mesothelium	Present	Regionally restricted		Expression is detected in the epithelium bounding the coelom, particularly over the (outer) parietal lining, and the mesothelium of the heart and gut.
TS18: pericardium	Present	Regionally restricted		Expression is detected in the epithelium bounding the coelom, particularly over the (outer) parietal lining, and the mesothelium of the heart and gut.

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Present

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Weak	Regionally restricted		Little sign of expression except in the involuting glomeruli.
TS21: metanephros	Strong	Regionally restricted		Strong over whole of the developing renal vesicle and weak over stem cells of the developing kidney.
TS21: spinal cord	Present	Regionally restricted		Present over whole length of spinal cord but more diffuse than in earlier stages and extends more toward ventral region of marginal layer.
TS21: intraembryonic coelom	Present	Regionally restricted		Expression is detected over the mesothelial linings of the major organs.