Efna2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type:	RNA in situ
MGI Accession ID:	MGI:2148280
Gene symbol:	Efna2
Gene name:	ephrin A2

Probe:	Elf-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

2Q ©

Specimen 2Q: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2Q	High levels in caudal mesencephalon; low levels in anterior mesencephalon.

2R ©

Specimen 2R: embryonic day 11.5; Emx2^tm1Sia/Emx2^tm1Sia, Otx2^tm1Sia/Otx2⁺ (more

)
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2R	Authors report normal patterns of expression in double mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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