Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64588
Toresson H, et al., Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for pax6 and gsh2. Development. 2000 Oct;127(20):4361-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2150370
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Gene symbol:
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Dlx1
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Gene name:
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distal-less homeobox 1
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Antibody:
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anti-distal-less
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Detection system:
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Secondary antibody
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Assay notes:
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Antibody is reported to be cross-reactive amongst phyla and recognizes at least five vertebrate Dlx proteins. Authors indicate detection of mouse proteins for Dlx1, 2, 5 and 6 genes; all have been annotated.
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Results
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Specimen
2A:
embryonic day 11.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2A
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High levels in lateral ganglionic eminence (LGE) progenitors.
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Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2G
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Reduced domain of expression; only in a few ventral LGE cells; missing from large portion of LGE.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Authors report alterations in expression in the LGE; expression in almost half of LGE.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Ectopic expression in germinal zone of cortex.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Expression throughout LGE and into ventrolateral cortex.
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Specimen
6A:
embryonic day 14.0
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
6A
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Expressed ventrally.
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Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
6F
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Ectopic expression in cortical progenitors throughout much of cortex, mainly in the subventricular zone (SVZ) and IZ.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
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Expression in progenitors throughout the LGE up to the LGE/cortex boundary.
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Specimen
7E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
7E
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Ectopic expression in LGE progenitors; same domain as in Sey mutant, but in fewer cells.
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
7F
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Ectopic expression in LGE progenitors; somewhat reduced levels compared with Sey/Sey.
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Analysis Tools
