Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:71879
Bernier G, et al., Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development. 2001 Oct;128(20):3987-94
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2153752
|
|
Gene symbol:
|
Lhx2
|
|
Gene name:
|
LIM homeobox protein 2
|
|
Probe:
|
Lhx2 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2J:
embryonic day 9.5
(more  )
Specimen
2J:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
|
2J
|
Predomiant expression in the inner layer of the optic vesicle.
|
Specimen
4D, +/+:
embryonic day 9.5
(more )
Specimen
4D, +/+:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4D, -/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Pax6tm1Pgr/Pax6tm1Pgr
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: embryo |
Present |
|
4D -/-
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
Not shown:
embryonic day 17.5
(more )
Specimen
Not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: ciliary body |
Present |
|
|
Expression in the ciliary margin of the retina.
|
|
Analysis Tools