Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72725
Martinez-Barbera JP, et al., Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development. 2001 Dec;128(23):4789-800
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2155488
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.7
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future midbrain |
Present |
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2A
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This result is inferred by a GXD curator based on the discussion in the text.
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Specimen
2O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
2O
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The expression domain is caudalized in this mutant.
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Specimen
4A:
embryonic day 9.7
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
4A
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Expression is on the mesencephalic side of the midbrain-hindbrain junction.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1+
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
4F
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The posterior border of expression is displaced anteriorly in the lateral regionof the caudal midbrain.
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Specimen
5A:
embryonic day 7.5
(more )
Note: Embryo is in the headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
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Specimen
5A'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: future brain |
Present |
Regionally restricted |
5A''
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
5C:
embryonic day 8.0
(more )
Note: Embryo is in the late headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the late headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
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Specimen
5C'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the late headfold stage. Also stained for Hoxb1 (assay MGI:3655643).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5C''
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In this mutant the region of expression expands posteriorly and fuses with the Hoxb1 expression domain.
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Specimen
5E:
embryonic day 8.0
(more )
Note: Embryo is in the presomite stage. Also stained for Hoxb1 (assay MGI:3655643).
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the presomite stage. Also stained for Hoxb1 (assay MGI:3655643).
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Specimen
5E'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the presomite stage. Also stained for Hoxb1 (assay MGI:3655643).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5E''
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In this mutant the region of expression expands posteriorly and fuses with the Hoxb1 expression domain.
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Specimen
5G:
embryonic day 8.0
(more )
Note: 1-2 somite stage.
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1-2 somite stage.
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Specimen
5G'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1-2 somite stage.
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Specimen
5I:
embryonic day 8.0
(more )
Note: 4-5 somite stage.
Specimen
5I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-5 somite stage.
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Specimen
5I'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-5 somites.
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Specimen
7A:
embryonic day 8.7
(more )
Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
7K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
7K
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The results of this assay are not discussed in the text. However, the domains of expression are clearly different than in the wild-type embryo.
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Analysis Tools