Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2159414
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 Probe12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Heterozygote Nodal mutants at E8.5 are defined as Type I, II or III mutants depending on the penetrance of the phenotype.
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Results
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Specimen
3. K.:
embryonic day 7.0
(more  )
Specimen
3. K.:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3. L.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
3L
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Expression is detected in the anterior ectoderm and the visceral endoderm but is more distal than in wild-type.
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| TS10: extraembryonic visceral endoderm |
Present |
Regionally restricted |
3L
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Expression is detected in the anterior ectoderm and the visceral endoderm but is more distal than in wild-type.
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Specimen
3. M.:
embryonic day 7.5
(more )
Specimen
3. M.:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3. N.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo ectoderm |
Present |
Regionally restricted |
3N
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Expression is detected in the anterior ectoderm and the visceral endoderm but is more distal than in wild-type.
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| TS11: extraembryonic visceral endoderm |
Present |
Regionally restricted |
3N
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Expression is detected in the anterior ectoderm and the visceral endoderm but is more distal than in wild-type.
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Specimen
4. H.:
embryonic day 8.5
(more )
Specimen
4. H.:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4. I.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type I mutant.
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Specimen
4. J.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type II mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head |
Weak |
Regionally restricted |
4J
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Expression is reduced in the head as compared to wild-type and is restricted to a small anterior domain.
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Specimen
4. K.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type III mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head |
Weak |
Regionally restricted |
4K
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Expression is reduced in the head as compared to wild-type and is restricted to the extreme anterior.
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