Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2159421
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Gene symbol:
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Hesx1
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Gene name:
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homeobox gene expressed in ES cells
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Probe:
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Hesx1/Rpx probe7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3. O.:
embryonic day 7.5
(more  )
Specimen
3. O.:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3. P.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: anterior visceral endoderm |
Present |
Regionally restricted |
3P
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Expression is detected in the anterior visceral endoderm and the overlying neural plate but is more distal than in wild-type.
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| TS11: future spinal cord neural plate |
Present |
Regionally restricted |
3P
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Expression is detected in the anterior visceral endoderm and the overlying neural plate but is more distal than in wild-type.
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Analysis Tools