Gene Expression Data
Assay Details
Assay
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Reference:
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J:63215
Schwitzgebel VM, et al., Expression of neurogenin3 reveals an islet cell precursor population in the pancreas. Development. 2000 Aug;127(16):3533-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2386026
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Gene symbol:
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Neurog3
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Gene name:
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neurogenin 3
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Antibody:
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anti-Neurogenin3
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
3A:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Ins1 (assay MGI:2386029) and Ins2 (assay MGI:2386037).
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Neurog3; red - Ins1 (assay MGI:2386029) and Ins2 (assay MGI:2386037).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
|
3A
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Authors report no co-staining with insulin.
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Specimen
3B:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Gcg (assay MGI:2386044).
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
Double labeled: green - Neurog3; red - Gcg (assay MGI:2386044).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
|
3B
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Authors report no co-staining with glucagon.
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Specimen
3C:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Pyy (assay MGI:2386046).
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Neurog3; red - Pyy (assay MGI:2386046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
|
3C
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Authors report no co-staining with peptide YY.
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Specimen
4A:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Nkx2-2 (assay MGI:2386047).
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Neurog3; red - Nkx2-2 (assay MGI:2386047).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
|
4A
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Authors report co-staining with islet cell precursor marker Nkx2.2
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Specimen
4C:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Isl1 (assay MGI:2386049).
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Neurog3; red - Isl1 (assay MGI:2386049).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
|
4C
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Authors report no co-staining with Isl1,are normally present in fully differentiated islet cells;
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Specimen
4E:
embryonic day 15.5
(more )
Note: Double labeled: green - Neurog3; red - Pdx1 (assay MGI:2386051).
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Neurog3; red - Pdx1 (assay MGI:2386051).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
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4E
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Authors report no co-staining with Pdx1, restricted to beta and gamma cells.
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