Gene Expression Data
Assay Details
Assay
|
Reference:
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J:67304
Bulchand S, et al., LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Mech Dev. 2001 Feb;100(2):165-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2386913
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Gene symbol:
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Wnt2b
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Gene name:
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wingless-type MMTV integration site family, member 2B
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Probe:
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clone PRK
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1a:
embryonic day 12.5; Lhx2tm1Dra/Lhx2 +
(more )
Specimen
1a:
(close )
Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
1a
|
Expression at medial edge of dorsal telencephalon, marking the cortical hem.
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Specimen
1b:
(close )
Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
|
embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
1b
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Compared to controls, expression consistently detected in a broader stretch of dorsal telencephalic tissue, on lateral side.
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Specimen
1g:
embryonic day 12.5
(more )
Specimen
1g:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
1g
|
Expression at medial edge of dorsal telencephalon, marking cortical hem. Authors report expression pattern similar to that seen in +/- embryos.
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Specimen
1h:
embryonic day 12.5
(more )
Specimen
1h:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
1h
|
Expression at medial edge of dorsal telencephalon, marking cortical hem. Authors report expression pattern similar to that seen in +/- embryos.
|
Specimen
3g:
embryonic day 15.5
(more )
Specimen
3g:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3g
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Expression in the fimbria, remnant of the cortical hem.
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Specimen
3h:
(close )
Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3h
|
Expression in cortical hem, dominating the dorsal telencephalon for much of its rostro-caudal extent.
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Specimen
3i:
embryonic day 15.5
(more )
Specimen
3i:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3i
|
Expression in the fimbria, remnant of the cortical hem.
|
Specimen
3j:
(close )
Genetic Background:
|
involves: 129S4/SvJae
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Mutant Allele(s):
|
Lhx2tm1Dra/Lhx2tm1Dra
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3j
|
Expression in cortical hem, dominating the dorsal telencephalon for much of its rostro-caudal extent.
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Specimen
3k:
embryonic day 15.5
(more )
Specimen
3k:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3k
|
Expression in cortical hem, dominating the dorsal telencephalon for much of its rostro-caudal extent.
|
Specimen
5b:
embryonic day 12.5
(more )
Specimen
5b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
5b
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Expression restricted to cortical hem.
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