Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67304
Bulchand S, et al., LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Mech Dev. 2001 Feb;100(2):165-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2386924
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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SHH cDNA17
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4c:
embryonic day 12.5
(more  )
Specimen
4c:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: choroid invagination |
Present |
Regionally restricted |
4c
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Authors report comparable expression pattern to that seen in control embryos
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Specimen
4d:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4d
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Authors report expression pattern comparable to that seen in control embryos.
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Analysis Tools