Probe:	Figf probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: extraembryonic component	Present	Regionally restricted	1A/A'	Expression is in the decidua basalis, laterally excluding the vascular zone.
TS28: decidua	Present	Regionally restricted	1A/A'	Expression is in the decidua basalis, laterally excluding the vascular zone.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1B

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1C

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		1D/D'

Structure	Level	Pattern	Image	Note
TS17: trunk sclerotome	Present	Regionally restricted	1E/E'	Expression is in the less condensed subregion.
TS17: 1st branchial arch mandibular component	Present		1E/E'
TS17: 1st branchial arch maxillary component	Present		1E/E'
TS17: 2nd branchial arch	Present	Regionally restricted	1E/E'
TS17: 3rd branchial arch	Present	Regionally restricted	1E/E'

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		1F/F'
TS17: 1st branchial arch maxillary component	Present		1F/F'
TS17: 2nd branchial groove	Present		1F/F'
TS17: acoustic ganglion	Present		1F/F'
TS17: limb mesenchyme	Present		1F/F'
TS17: medial-nasal process mesenchyme	Present		1F/F'
TS17: 2nd branchial arch	Present	Regionally restricted	1F/F'
TS17: 3rd branchial arch	Present	Regionally restricted	1F/F'

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Present		1G/G'	Expression in cells delaminating from Rathke's pouch, corresponding to the anterior pituitary primordium.
TS20: liver	Present		1G/G'

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process	Weak
TS20: limb	Weak

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present
TS20: utricle	Present

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Present		1H/H'	Expression in cells delaminating from Rathke's pouch, corresponding to the anterior pituitary primordium.

Structure	Level	Pattern	Image	Note
TS25: hepatic artery	Strong		1I/I'	Authors report expression in hepatic blood vessels.
TS25: hepatic vein	Strong		1I/I'	Authors report expression in hepatic blood vessels.
TS25: adrenal gland	Strong		1I/I'
TS25: bladder	Strong		1I/I'
TS25: liver	Strong		1I/I'
TS25: lower jaw tooth	Strong		1I/I'
TS25: lung	Strong		1I/I'
TS25: metanephros	Strong		1I/I'
TS25: skin	Strong		1I/I'
TS25: upper jaw tooth	Strong		1I/I'
TS25: axial skeleton	Strong	Regionally restricted	1I/I'	Expression is in the periosteum of the vertebral bodies.

Structure	Level	Pattern	Image	Note
TS25: bladder	Present		1J/J'
TS25: hyoglossus	Present		1J/J'
TS25: skin	Present		1J/J'
TS25: tooth	Present		1J/J'

Structure	Level	Pattern	Image	Note
TS25: inner ear	Absent

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present		2A/A'
TS17: apical ectodermal ridge	Absent		2A/A'

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		2B/B'

Structure	Level	Pattern	Image	Note
TS20: vestibular ganglion	Strong		2C/C'

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Present		2D/D'

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Strong		2E/E'

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		2F/F'	Sharp boundary of expression between par anterior and Rathke's pouch cells.
TS20: remnant of Rathke's pouch	Absent		2F/F'	Sharp boundary of expression between par anterior and Rathke's pouch cells.

Structure	Level	Pattern	Image	Note
TS25: adenohypophysis pars anterior	Present	Regionally restricted	2G/G'
TS25: adenohypophysis pars tuberalis	Present	Graded	2G/G'	Mostly surrounding the infundibular stalk; gradually decreasing toward opposite site.

Structure	Level	Pattern	Image	Note
TS25: adrenal gland	Strong		2H/H'
TS25: metanephros	Present	Regionally restricted	2H/H'	Authors state that expression is in the stromal mesenchyme, excluding connecting tubules and glomeruli.
TS25: glomerular tuft	Absent		2H/H'
TS25: renal capsule	Present		2H/H'

Structure	Level	Pattern	Image	Note
TS25: axial skeleton	Present	Regionally restricted	2I/I'	Expression is in the periosteum of the vertebral bodies.

Structure	Level	Pattern	Image	Note
TS25: tooth mesenchyme	Present	Regionally restricted	2J/J'	Expression is close to the inner epithelium and at a lower level adjacent to the outer enamel epithelium.

Structure	Level	Pattern	Image	Note
TS25: skin	Present	Regionally restricted	2K/K'	Expression is in the mesenchyme surrounding germinal cells having proliferative and differentiative properties.

Structure	Level	Pattern	Image	Note
TS25: lung mesenchyme	Present	Regionally restricted	2L/L'	Expression is confined to the connective tissue.
TS25: left lung bronchiole	Absent		2L/L'
TS25: right lung accessory lobe bronchiole	Absent		2L/L'