Probe:	Gdf5 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Specimens were fixed after sectioning in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	1A	Expression is in a region where the shoulder and elbow will later form.

Structure	Level	Pattern	Image	Note
TS19: handplate	Weak	Regionally restricted		Interdigital expression and expression in the palmar arch.

Structure	Level	Pattern	Image	Note
TS20: shoulder	Present		1D
TS20: hand	Strong	Regionally restricted	1D	Expression within the developing digital ray. This site coincides with the region of the future joint between the metacarpals and the proximal phalanges. Expression extends proximally along the outer perimeter of the metacarpal.

Structure	Level	Pattern	Image	Note
TS20: elbow	Present
TS20: hand	Present	Regionally restricted		Expression in a region of the developing carpals.
TS20: forelimb interdigital region	Weak

Structure	Level	Pattern	Image	Note
TS21: elbow joint primordium	Present		1G
TS21: shoulder joint primordium	Present		1G

Structure	Level	Pattern	Image	Note
TS21: hand	Weak	Regionally restricted		Expression around the metacarpals.
TS21: forelimb interdigital region mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present	Regionally restricted	1H	Expression at the presumptive joint region between the metacarpals and the proximal phalanges, and where the first interphalangeal joint will form.
TS21: hand mesenchyme	Present	Regionally restricted	1H	Expression between the developing rows of carpals (developing joints of the wrist).

Structure	Level	Pattern	Image	Note
TS22: forelimb digit mesenchyme	Present	Regionally restricted	1I	A third stripe of expression in the digital ray corresponding to the site where the second interphalangeal joint will form (as well as the same sites of expression as in 1H).

Structure	Level	Pattern	Image	Note
TS22: elbow joint primordium	Present

Structure	Level	Pattern	Image	Note
TS23: forelimb	Weak			Expression decreases after this age, the authors state that it is possible that expression continues, however due to increased background staining it is hard to detect.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present		6B
TS22: sternum	Present		6B

Structure	Level	Pattern	Image	Note
TS22: T2 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T3 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T4 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T5 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T6 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T7 rib cartilage condensation	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: sternum	Present	Regionally restricted	6D	Expression where the rib contacts the sternum.
TS22: T1 rib cartilage condensation	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: T4 rib cartilage condensation	Present	Regionally restricted	6F	Expression where the rib contacts the sternum.
TS22: sternum	Present	Regionally restricted	6F	Expression where the rib contacts the sternum.