Gene Expression Data
Assay Details
Assay
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Reference:
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J:81302
Fleming TP, et al., Localisation of tight junction protein cingulin is temporally and spatially regulated during early mouse development. Development. 1993 Mar;117(3):1135-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2450133
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Gene symbol:
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Cgn
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Gene name:
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cingulin
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Antibody:
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anti-Cingulin
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Detection system:
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Secondary antibody
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Assay notes:
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For whole mount specimens, the fixative was 0.5% formaldehyde for 20 minutes. For sectioned specimens, the fixative was 1% formaldehyde.
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Results
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Specimen
1A:
postnatal adult
(more )
Note: Egg was freed from cumulus cells and zona pellucida.
Specimen
1A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Egg was freed from cumulus cells and zona pellucida.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
1A
,
1B
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Expression was detected in the cytoplasmic face of the oolemma in 100% of oocytes. In the cytocortex, the microvillous domain was stained, but staining was absent or poor in the smooth membrane domain overlying the metaphase II spindle.
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Specimen
1D:
postnatal adult
(more )
Note: Egg was freed from cumulus cells and zona pellucida.
Specimen
1D:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Egg was freed from cumulus cells and zona pellucida.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
1D
,
1C
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Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.
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Specimen
1F:
postnatal adult
(more )
Note: Egg was freed from cumulus cells and zona pellucida.
Specimen
1F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Note:
|
Egg was freed from cumulus cells and zona pellucida.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
1E
,
1F
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Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.
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Specimen
Not Shown:
embryonic day 0.5
(more )
Note: Fertilized egg.
Specimen
Not Shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 0.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Fertilized egg.
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Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
Regionally restricted |
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Expression was detected in the cytoplasmic face of the oolemma of 100% of the fertilized eggs.
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Specimen
Not Shown:
embryonic day 1.0
(more )
Note: 2-cell embryo.
Specimen
Not Shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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2-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
Regionally restricted |
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Expression was detected in the cytocortex either uniformly or preferentially on the outer surface of 69% of embryos.
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Specimen
2A:
embryonic day 2.0
(more )
Note: 4-cell embryo.
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 2.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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4-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
Regionally restricted |
2A
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Expression was detected in the cytocortex either uniformly or preferentially on the outer surface of 78% of embryos.
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Specimen
Not Shown:
embryonic day 2.5
(more )
Note: Precompact 8-cell embryo.
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Precompact 8-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage embryo |
Present |
Regionally restricted |
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Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 57% of the embryos but absent from the nascent apicolateral tight junctions site.
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Specimen
2B:
embryonic day 2.5
(more )
Note: Compact 8-cell embryo.
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Compact 8-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
Regionally restricted |
2B
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Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 85% of the embryos but absent from the nascent apicolateral tight junctions site.
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Specimen
2C:
embryonic day 3.0
(more )
Note: 16-cell embryo.
Specimen
2C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 3.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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16-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
Regionally restricted |
2C
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Expression in the cytocortex was in the apical microvillous pole in outer blastomeres of the trophectoderm lineage of 67% of embryos. Faint zonular or discontinuous staining was in the forming tight junction sites of each blastomere in 75% of embryos.
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Specimen
Not Shown:
embryonic day 3.5
(more )
Note: Late morula embryo.
Specimen
Not Shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Note:
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Late morula embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Regionally restricted |
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Expression was absent from the inner cell mass and the cytocortex, but was present in the tight junctions in the trophectoderm in 100% of embryos and in punctate cytoplasmic foci surrounding the trophectoderm nuclei in 70% of embryos.
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