Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50953
Jiang S, et al., Strain-dependent developmental relaxation of imprinting of an endogenous mouse gene, Kvlqt1. Genomics. 1998 Nov 1;53(3):395-9
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2659125
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Gene symbol:
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Kcnq1
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Gene name:
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potassium voltage-gated channel, subfamily Q, member 1
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Probe:
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U1, L1
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Assay notes:
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After RT-PCR analyses, the bands were extracted and digested with BsmFI which cleaves the CAST/Ei allele into a 140 and 97 bp fragments. This assay indicates complete relaxation of imprinting in tissues. Both alleles are expressed equally.
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Results
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Image: 4c
| Control |
| Control |
| P adult
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TS28: mouse
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Present
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Absent
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1 µg; total RNA |
129S/SvEv |
| Male |
Specific tissues were not detailed.
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| P adult
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TS28: mouse
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Absent
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Present
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1 µg; total RNA |
CAST/EiJ |
| Female |
Specific tissues were not detailed.
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| P adult
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TS28: metanephros
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: spleen
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: liver
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: brain
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: intestine
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: heart
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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| P adult
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TS28: lung
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Present
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Present
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1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
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Notes:
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Analysis Tools
