Antibody:	anti-Slit2 (E-20)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		2A
TS20: lung epithelium	Present	Regionally restricted	2A	Expression in the cell apices adjacent to the bronchiolar lumen.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		2B
TS20: lung epithelium	Present	Regionally restricted	2B	Expression in the cell apices adjacent to the bronchiolar lumen.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		2C
TS22: lung epithelium	Present	Regionally restricted	2C	Expression in the cell apices adjacent to the bronchiolar lumen.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		2D
TS22: lung epithelium	Present	Regionally restricted	2D	Expression in the cell apices adjacent to the bronchiolar lumen.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present		3A
TS24: lung epithelium	Strong	Regionally restricted	3A	Expression at the apical surface of the bronchiolar epithelium.
TS24: lung	Present	Regionally restricted	3A	Protein was found in the airway lumen.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present		3B
TS24: lung epithelium	Strong	Regionally restricted	3B	Expression at the apical surface of the bronchiolar epithelium.
TS24: lung	Present	Regionally restricted	3B	Protein was found in the airway lumen.

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Present	Regionally restricted	3C	Expression in the saccular mesenchyme.
TS26: lung epithelium	Strong	Regionally restricted	3C	Expression surrounding the large airways.

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Present	Regionally restricted	3D	Expression in the saccular mesenchyme.
TS26: lung epithelium	Strong	Regionally restricted	3D	Expression surrounding the large airways.