Antibody:	anti-Calretinin
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Single cells	6D Control	Expression was detected in neurons and neuronal processes within the subplate.
TS26: cerebral cortex marginal zone	Present	Single cells	6D Control	Expression was detected in neurons, Cajal-Retzius cells and neuronal processes within the marginal zone.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Weak	Single cells	6E Emx2-/-	There was a marked reduction in the number and complexity of immunopositive neurons and neuronal processes within the subplate of mutants, particularly in the anterior to mid-cortex.
TS26: cerebral cortex marginal zone	Weak	Single cells	6E Emx2-/-	There was a marked reduction in the number and complexity of immunopositive neurons, Cajal-Retzius cells and neuronal processes within the marginal zone of mutants, particularly in the anterior to mid-cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Single cells	7E	Immunopositive neurons and processes were detected in heterotopias in the mutant cortex.
TS27: meninges	Present	Single cells	7E	Immunopositive neurons and processes were detected in heterotopias in the mutant cortex.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex mantle layer	Present	Single cells	8A part (a) wt , 8B part (i) wt post. , 8B part (c) wt ant.	Expression was detected in the neurons and neuronal processes within the subplate.
TS21: cerebral cortex marginal layer	Present	Single cells	8A part (a) wt , 8B part (i) wt post. , 8B part (c) wt ant.	Expression was detected in neurons, Cajal-Retzius cells and neuronal processes within the marginal zone.